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Literature summary extracted from

  • Al-Shareffi, E.; Chaubard, J.L.; Leonhard-Melief, C.; Wang, S.K.; Wong, C.H.; Haltiwanger, R.S.
    6-Alkynyl fucose is a bioorthogonal analog for O-fucosylation of epidermal growth factor-like repeats and thrombospondin type-1 repeats by protein O-fucosyltransferases 1 and 2 (2013), Glycobiology, 23, 188-198.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.221 Mus musculus
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-
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.221 GDP-6-alkynyl fucose + protein 6-alkynyl fucose is efficiently incorporated onto EGF repeats, TSRs, and N-glycan on Lfng and N-glycans on a number of proteins in crude lysates of CHO cells, e.g. the O-fucosylation site in EGF3 of mouse Notch1 and elongated by Lfng, mass spectrometry analysis, overview. Using the Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC), or click reaction, azido-biotin allows tagging and detection of 6AF-modified proteins Mus musculus ?
-
 ?
2.4.1.221 GDP-fucose + TSR1
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 Mus musculus GDP + fucosyl-TSR1
-
 ?
2.4.1.221 GDP-fucose + TSR2
-
 Mus musculus GDP + fucosyl-TSR2
-
 ?
2.4.1.221 GDP-fucose + TSR3
-
 Mus musculus GDP + fucosyl-TSR3
-
 ?
2.4.1.221 additional information protein O-fucosyltransferase 1 and protein O-fucosyltransferase 2 add O-linked fucose at distinct consensus sequences in properly folded epidermal growth factor (EGF)-like repeats and thrombospondin type-1 (TSR) repeats, respectively Mus musculus ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.221 Pofut1
-
 Mus musculus
2.4.1.221 POFUT2
-
 Mus musculus
2.4.1.221 protein O-fucosyltransferases 1
-
 Mus musculus
2.4.1.221 protein O-fucosyltransferases 2
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.4.1.221 malfunction elimination of Pofut1 in mice causes embryonic lethality with Notch-like phenotypes, elimination of Pofut2 results in an early embryonic lethal phenotype in mice Mus musculus
2.4.1.221 physiological function O-fucosylation of Notch is essential for its function Mus musculus