EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.251 | dodecylmaltoside | maximal activity in the presence of dodecylmaltoside at a concentration of 33.3 critical micelle concentration | Xanthomonas campestris | |
2.4.1.251 | Triton X-100 | maximal activity at 133 critical micelle concentration or 32 mM or 2%, representing about 45% of that observed with dodecylmaltoside | Xanthomonas campestris |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.251 | expression in Escherichia coli | Xanthomonas campestris |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.251 | membrane | monotopic membrane protein | Xanthomonas campestris | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.251 | 36000 | - |
x * 36000, SDS-PAGE, recombinant protein | Xanthomonas campestris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.251 | Xanthomonas campestris | Q56775 | - |
- |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.251 | -20°C, purified protein at 5 mg/ml is stable for at least 5 months | Xanthomonas campestris |
2.4.1.251 | 4°C, purified protein at 5 mg/ml is stable for at least 1 month at 4°C | Xanthomonas campestris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.251 | ADP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | ADP-mannose can act as a mannose donor, yielding around 35% of the reaction product obtained by using GDP-mannose | Xanthomonas campestris | ADP + D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | - |
? | |
2.4.1.251 | GDP-alpha D-glucose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | less than 15% of the reaction product obtained by using GDP-mannose | Xanthomonas campestris | GDP + D-Glc-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | - |
? | |
2.4.1.251 | GDP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | enzyme is a nonprocessive beta-mannosyltransferase catalyzing the transfer of a mannose residue from GDP-Man to glucuronic acid-beta-1,2-mannose-alpha-1,3-glucose-beta-1,4-glucose-PP-polyisoprenyl to form the lipid-linked pentasaccharide | Xanthomonas campestris | GDP + D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.251 | ? | x * 36000, SDS-PAGE, recombinant protein | Xanthomonas campestris |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.251 | gumI | - |
Xanthomonas campestris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.251 | 35 | - |
- |
Xanthomonas campestris |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.251 | 20 | - |
about 80% of maximum activity | Xanthomonas campestris |
2.4.1.251 | 60 | - |
almost complete loss of activity | Xanthomonas campestris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.251 | 8 | - |
- |
Xanthomonas campestris |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.251 | 7.5 | 9.5 | - |
Xanthomonas campestris |