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Literature summary extracted from

  • Salinas, S.R.; Bianco, M.I.; Barreras, M.; Ielpi, L.
    Expression, purification and biochemical characterization of GumI, a monotopic membrane GDP-mannose:glycolipid 4-beta-D-mannosyltransferase from Xanthomonas campestris pv. campestris (2011), Glycobiology, 21, 903-913.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.251 dodecylmaltoside maximal activity in the presence of dodecylmaltoside at a concentration of 33.3 critical micelle concentration Xanthomonas campestris
2.4.1.251 Triton X-100 maximal activity at 133 critical micelle concentration or 32 mM or 2%, representing about 45% of that observed with dodecylmaltoside Xanthomonas campestris

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.251 expression in Escherichia coli Xanthomonas campestris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.251 membrane monotopic membrane protein Xanthomonas campestris 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.251 36000
-
 x * 36000, SDS-PAGE, recombinant protein Xanthomonas campestris

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.251 Xanthomonas campestris Q56775
-
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.251 -20°C, purified protein at 5 mg/ml is stable for at least 5 months Xanthomonas campestris
2.4.1.251 4°C, purified protein at 5 mg/ml is stable for at least 1 month at 4°C Xanthomonas campestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.251 ADP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol ADP-mannose can act as a mannose donor, yielding around 35% of the reaction product obtained by using GDP-mannose Xanthomonas campestris ADP + D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol
-
 ?
2.4.1.251 GDP-alpha D-glucose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol less than 15% of the reaction product obtained by using GDP-mannose Xanthomonas campestris GDP + D-Glc-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol
-
 ?
2.4.1.251 GDP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol enzyme is a nonprocessive beta-mannosyltransferase catalyzing the transfer of a mannose residue from GDP-Man to glucuronic acid-beta-1,2-mannose-alpha-1,3-glucose-beta-1,4-glucose-PP-polyisoprenyl to form the lipid-linked pentasaccharide Xanthomonas campestris GDP + D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.251 ? x * 36000, SDS-PAGE, recombinant protein Xanthomonas campestris

Synonyms

EC Number Synonyms Comment Organism
2.4.1.251 gumI
-
 Xanthomonas campestris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.251 35
-
-
 Xanthomonas campestris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.251 20
-
 about 80% of maximum activity Xanthomonas campestris
2.4.1.251 60
-
 almost complete loss of activity Xanthomonas campestris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.251 8
-
-
 Xanthomonas campestris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.251 7.5 9.5
-
 Xanthomonas campestris