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Literature summary extracted from

  • Both, P.; Sobczak, L.; Breton, C.; Hann, S.; Noebauer, K.; Paschinger, K.; Kozmon, S.; Mucha, J.; Wilson, I.B.
    Distantly related plant and nematode core alpha1,3-fucosyltransferases display similar trends in structure-function relationships (2011), Glycobiology, 21, 1401-1415.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.214 expressed in Pichia pastoris strain GS115 Caenorhabditis elegans
2.4.1.214 expressed in Pichia pastoris strain GS115 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.214 D303E the mutant retains 23.8% of the wild type activity Caenorhabditis elegans
2.4.1.214 N194Q glycosylation site mutant with less than 1% of wild type activity Caenorhabditis elegans
2.4.1.214 N219A the mutant retains 1.2% of the wild type activity Arabidopsis thaliana
2.4.1.214 N337A the mutant retains 18.6% of the wild type activity Arabidopsis thaliana
2.4.1.214 R226A the mutation leads to a complete loss of activity Arabidopsis thaliana
2.4.1.214 S218A the mutation completely abolishes the enzyme activity Arabidopsis thaliana
2.4.1.214 S243A the mutant retains 16.2% of the wild type activity Caenorhabditis elegans
2.4.1.214 S253A the mutant retains 35.6% of the wild type activity Arabidopsis thaliana
2.4.1.214 S483A the mutant retains 32.2% of the wild type activity Arabidopsis thaliana
2.4.1.214 T339A the mutant retains 12.1% of the wild type activity Arabidopsis thaliana
2.4.1.214 T361A the mutant retains 24.3% of the wild type activity Caenorhabditis elegans
2.4.1.214 T422A the mutant retains 36.5% of the wild type activity Arabidopsis thaliana
2.4.1.214 Y243A the mutant retains 9.8% of the wild type activity Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.214 Cu2+ 3% residual activity in the presence of 1 mM Arabidopsis thaliana
2.4.1.214 Cu2+ 3% residual activity in the presence of 1 mM Caenorhabditis elegans
2.4.1.214 EDTA about 5% residual activity in the presence of 10 mM Arabidopsis thaliana
2.4.1.214 EDTA about 5% residual activity in the presence of 10 mM Caenorhabditis elegans
2.4.1.214 Zn2+ 3% residual activity in the presence of 1 mM Arabidopsis thaliana
2.4.1.214 Zn2+ 3% residual activity in the presence of 1 mM Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.214 0.1
-
 GDP-beta-L-fucose mutant enzyme S253A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C Arabidopsis thaliana
2.4.1.214 0.24
-
 GDP-beta-L-fucose mutant enzyme Y243A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C Arabidopsis thaliana
2.4.1.214 0.33
-
 GDP-beta-L-fucose mutant enzyme N219A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C Arabidopsis thaliana
2.4.1.214 0.36
-
 GDP-beta-L-fucose mutant enzyme T339A, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C Arabidopsis thaliana
2.4.1.214 1
-
 GDP-beta-L-fucose wild type enzyme, 10 mM MnCl2, 50 mM MES buffer, pH 6.5, at 30°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.214 Ca2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Caenorhabditis elegans
2.4.1.214 Ca2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Arabidopsis thaliana
2.4.1.214 Co2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Caenorhabditis elegans
2.4.1.214 Co2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Arabidopsis thaliana
2.4.1.214 Mg2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Caenorhabditis elegans
2.4.1.214 Mg2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Arabidopsis thaliana
2.4.1.214 Mn2+ highest relative enzyme activity in the presence of Mn2+ Caenorhabditis elegans
2.4.1.214 Mn2+ highest relative enzyme activity in the presence of Mn2+ Arabidopsis thaliana
2.4.1.214 additional information Zn2+ and Cu2+ ions do not activate the enzyme Caenorhabditis elegans
2.4.1.214 additional information Zn2+ and Cu2+ ions do not activate the enzyme Arabidopsis thaliana
2.4.1.214 Ni2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Caenorhabditis elegans
2.4.1.214 Ni2+ the enzyme is also active in the presence of Mg2+, Co2+, Ni2+ and Ca2+ Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.214 49000
-
 x * 49000, calculated from amino acid sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.214 GDP-beta-L-fucose + GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1-2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc Arabidopsis thaliana
-
 GDP + GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1->2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)(Fucalpha(1->3))GlcNAc
-
 ?
2.4.1.214 GDP-beta-L-fucose + Manalpha(1->6)[Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc Caenorhabditis elegans
-
 GDP + Manalpha(1->6)[Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->3)][L-Fucalpha(1->6)]GlcNAc
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.214 Arabidopsis thaliana Q9C8W3
-
-
2.4.1.214 Caenorhabditis elegans G5EDR5
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.214 glycoprotein
-
 Caenorhabditis elegans
2.4.1.214 glycoprotein
-
 Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.214 Ni-NTA or HisTrap column chromatography Caenorhabditis elegans
2.4.1.214 Ni-NTA or HisTrap column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.214 GDP-beta-L-fucose + GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1-2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc
-
 Arabidopsis thaliana GDP + GlcNAcbeta(1->2)Manalpha(1->6)(GlcNAcbeta(1->2)Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)(Fucalpha(1->3))GlcNAc
-
 ?
2.4.1.214 GDP-beta-L-fucose + GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc
-
 Arabidopsis thaliana GDP + GlcNAcbeta(1->2)Manalpha(1->6)[GlcNAcbeta(1->2)Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->3)][L-Fucalpha(1->6)]GlcNAc
-
 ?
2.4.1.214 GDP-beta-L-fucose + Manalpha(1->6)(Manalpha(1->3))Manalpha(1->6)(Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)GlcNAc
-
 Caenorhabditis elegans GDP + Manalpha(1->6)(Manalpha(1->3))Manalpha(1->6)(Manalpha(1->3))Manbeta(1->4)GlcNAcbeta(1->4)[Fucalpha(1->3)]GlcNAc
-
 ?
2.4.1.214 GDP-beta-L-fucose + Manalpha(1->6)[Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->6)]GlcNAc
-
 Caenorhabditis elegans GDP + Manalpha(1->6)[Manalpha(1->3)]Manbeta(1->4)GlcNAcbeta(1->4)[L-Fucalpha(1->3)][L-Fucalpha(1->6)]GlcNAc
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.214 ? x * 49000, calculated from amino acid sequence Arabidopsis thaliana
2.4.1.214 ? x * 60000-70000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.4.1.214 core alpha1,3-fucosyltransferase
-
 Caenorhabditis elegans
2.4.1.214 core alpha1,3-fucosyltransferase
-
 Arabidopsis thaliana
2.4.1.214 FucTA
-
 Arabidopsis thaliana
2.4.1.214 FUT-1
-
 Caenorhabditis elegans
2.4.1.214 FUT11
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.214 25 30
-
 Caenorhabditis elegans
2.4.1.214 30
-
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.214 6 7
-
 Arabidopsis thaliana
2.4.1.214 7.5
-
-
 Caenorhabditis elegans