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Literature summary extracted from

  • Liu, T.W.; Ito, H.; Chiba, Y.; Kubota, T.; Sato, T.; Narimatsu, H.
    Functional expression of L-fucokinase/guanosine 5-diphosphate-L-fucose pyrophosphorylase from Bacteroides fragilis in Saccharomyces cerevisiae for the production of nucleotide sugars from exogenous monosaccharides (2011), Glycobiology, 21, 1228-1236.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.7.30 synthesis functional expression of enzyme gene in Saccharomyces cerevisiae. The yeast cells synthesize GDP-L-fucose or GDP-D-arabinose upon exogenous addition of L-fucose or D-arabinose, respectively Bacteroides fragilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.52 functional expression of HA-tagged FKP in Saccharomyces cerevisiae strain W303-1A, the biosynthesis of GDP-D-Ara in the recombinant Saccharomyces cerevisiae strain harboring the FKP gene can occur through a mechanism akin to that of GDP-L-Fuc via the salvage pathway. Expression of a loss-of-function FKP mutant in yeast Bacteroides fragilis
2.7.7.30 expression in Saccharomyces cerevisiae Bacteroides fragilis

General Stability

EC Number General Stability Organism
2.7.1.52 instability of the enzyme occurs through chemical reduction in disulfide bonds of FKP protein, resulting in degradation Bacteroides fragilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.52 Mg2+ required Bacteroides fragilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.52 ATP + L-fucose Bacteroides fragilis
-
 ADP + beta-L-fucose 1-phosphate
-
 ?
2.7.1.52 ATP + L-fucose Bacteroides fragilis 9343
-
 ADP + beta-L-fucose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.52 Bacteroides fragilis
-
-
-
2.7.1.52 Bacteroides fragilis 9343
-
-
-
2.7.1.52 no activity in Saccharomyces cerevisiae
-
-
-
2.7.7.30 Bacteroides fragilis Q58T34
-
-
2.7.7.30 Bacteroides fragilis 9343 Q58T34
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.52 ATP + L-fucose
-
 Bacteroides fragilis ADP + beta-L-fucose 1-phosphate
-
 ?
2.7.1.52 ATP + L-fucose
-
 Bacteroides fragilis 9343 ADP + beta-L-fucose 1-phosphate
-
 ?
2.7.1.52 additional information fucokinase:GDP-fucose pyrophosphorylase is a dual activity enzyme metabolizing fucose and nucleotide-sugar Bacteroides fragilis ?
-
 ?
2.7.1.52 additional information fucokinase:GDP-fucose pyrophosphorylase is a dual activity enzyme metabolizing fucose and nucleotide-sugar Bacteroides fragilis 9343 ?
-
 ?
2.7.7.30 GTP + alpha-D-arabinopyranose 1-phosphate
-
 Bacteroides fragilis GDP-alpha-D-arabinopyranose + diphosphate
-
 ?
2.7.7.30 GTP + alpha-D-arabinopyranose 1-phosphate
-
 Bacteroides fragilis 9343 GDP-alpha-D-arabinopyranose + diphosphate
-
 ?
2.7.7.30 GTP + beta-L-fucose 1-phosphate
-
 Bacteroides fragilis GDP-L-fucose + diphosphate
-
 ?
2.7.7.30 GTP + beta-L-fucose 1-phosphate
-
 Bacteroides fragilis 9343 GDP-L-fucose + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.52 FKP
-
 Bacteroides fragilis
2.7.1.52 Fuk
-
 Bacteroides fragilis
2.7.1.52 L-fucokinase/guanosine 5'-diphosphate-L-fucose pyrophosphorylase
-
 Bacteroides fragilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.52 ATP
-
 Bacteroides fragilis

General Information

EC Number General Information Comment Organism
2.7.1.52 metabolism the enzyme catalyzes the first step of the salvage pathway converting exogenous L-Fuc to GDP-L-Fuc Bacteroides fragilis
2.7.1.52 physiological function the bifunctional L-Fuc pyrophosphorylase, FKP, converts L-Fuc into GDP-L-Fuc via an L-Fuc-1-phosphate intermediate Bacteroides fragilis