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Literature summary extracted from

  • Wang, N.; Shi, H.; Yao, Q.; Zhou, Y.; Kang, L.; Chen, H.; Chen, K.
    Cloning, expression and characterization of alcohol dehydrogenases in the silkworm Bombyx mori (2011), Genet. Mol. Biol., 34, 240-243.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged BmADH protein in Escherichia coli strain BL21 (DE3) Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 31000
-
 x * 31000, recombinant His6-tagged enzyme, SDS-PAGE Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.1 ethanol + NAD+ Bombyx mori
-
 acetaldehyde + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Bombyx mori Q1G151
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 soluble recombinant His6-tagged BmADH protein from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.1 egg
-
 Bombyx mori
-
1.1.1.1 epidermis
-
 Bombyx mori
-
1.1.1.1 fat body
-
 Bombyx mori
-
1.1.1.1 ganglion
-
 Bombyx mori
-
1.1.1.1 hemocyte
-
 Bombyx mori
-
1.1.1.1 hindgut
-
 Bombyx mori
-
1.1.1.1 malpighian tubule
-
 Bombyx mori
-
1.1.1.1 midgut
-
 Bombyx mori
-
1.1.1.1 additional information tissue expression profile of fifth instar larvae, overview Bombyx mori
-
1.1.1.1 ovary
-
 Bombyx mori
-
1.1.1.1 silk gland
-
 Bombyx mori
-
1.1.1.1 sperm
-
 Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 ethanol + NAD+
-
 Bombyx mori acetaldehyde + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.1 ? x * 31000, recombinant His6-tagged enzyme, SDS-PAGE Bombyx mori

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 ADH
-
 Bombyx mori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 25
-
 purified recombinant His-tagged enzyme, 10 min, completely stable up to Bombyx mori

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.1 8 9 purified recombinant His-tagged enzyme, 10 min, high stability within this range Bombyx mori

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Bombyx mori
1.1.1.1 NADH
-
 Bombyx mori