EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.87 | expression of His-tagged enzyme in Escherichia coli strain TOP 10F' | Candida albicans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.87 | (2R,3S)-3-(p-carboxybenzyl)malate | - |
Candida albicans | |
1.1.1.87 | (2S)-thiahomocitrate | - |
Candida albicans | |
1.1.1.87 | additional information | inhibitor docking study, molecular modeling of CaHIcDH-inhibitor interaction, overview | Candida albicans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.87 | 0.042 | - |
NAD+ | native enzyme, pH 7.8, 20°C | Candida albicans | |
1.1.1.87 | 0.074 | - |
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate | recombinant His-tagged enzyme, pH 7.8, 20°C | Candida albicans | |
1.1.1.87 | 0.45 | - |
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate | native enzyme, pH 7.8, 20°C | Candida albicans | |
1.1.1.87 | 1.09 | - |
NAD+ | recombinant His-tagged enzyme, pH 7.8, 20°C | Candida albicans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.87 | K+ | dependent on | Candida albicans | |
1.1.1.87 | Mg2+ | dependent on | Candida albicans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.87 | 41491 | - |
4 * 42600, SDS-PAGE, x * 41491, sequence calculation | Candida albicans |
1.1.1.87 | 42600 | - |
4 * 42600, SDS-PAGE, x * 41491, sequence calculation | Candida albicans |
1.1.1.87 | 158000 | - |
gel filtration | Candida albicans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.87 | (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+ | Candida albicans | strict specificity for homoisocitrate | 2-oxoadipate + CO2 + NADH + H+ | - |
? | |
1.1.1.87 | (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+ | Candida albicans ATCC 10231 | strict specificity for homoisocitrate | 2-oxoadipate + CO2 + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.87 | Candida albicans | - |
gene LYS12 | - |
1.1.1.87 | Candida albicans ATCC 10231 | - |
gene LYS12 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.87 | recombinant His-tagged enzyme from Escherichia coli strain TOP 10F by nickel affinity chromatography' | Candida albicans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.87 | (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+ | strict specificity for homoisocitrate | Candida albicans | 2-oxoadipate + CO2 + NADH + H+ | - |
? | |
1.1.1.87 | (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+ | strict specificity for homoisocitrate, the enzyme selectively binds the Mg(II):homoisocitrate complex | Candida albicans | 2-oxoadipate + CO2 + NADH + H+ | - |
? | |
1.1.1.87 | (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+ | strict specificity for homoisocitrate | Candida albicans ATCC 10231 | 2-oxoadipate + CO2 + NADH + H+ | - |
? | |
1.1.1.87 | (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+ | strict specificity for homoisocitrate, the enzyme selectively binds the Mg(II):homoisocitrate complex | Candida albicans ATCC 10231 | 2-oxoadipate + CO2 + NADH + H+ | - |
? | |
1.1.1.87 | additional information | substrate docking study | Candida albicans | ? | - |
? | |
1.1.1.87 | additional information | substrate docking study | Candida albicans ATCC 10231 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.87 | More | three-dimensional structure and homology modeling, overview | Candida albicans |
1.1.1.87 | tetramer | 4 * 42600, SDS-PAGE, x * 41491, sequence calculation | Candida albicans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.87 | HICDH | - |
Candida albicans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.87 | 20 | - |
assay at | Candida albicans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.87 | 0.38 | - |
NAD+ | recombinant His-tagged enzyme, pH 7.8, 20°C | Candida albicans | |
1.1.1.87 | 0.38 | - |
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate | recombinant His-tagged enzyme, pH 7.8, 20°C | Candida albicans | |
1.1.1.87 | 0.4 | - |
NAD+ | native enzyme, pH 7.8, 20°C | Candida albicans | |
1.1.1.87 | 0.4 | - |
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate | native enzyme, pH 7.8, 20°C | Candida albicans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.87 | 7.8 | - |
assay at | Candida albicans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.87 | NAD+ | - |
Candida albicans |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.1.1.87 | 0.000097 | - |
recombinant His-tagged enzyme, pH 7.8, 20°C | Candida albicans | (2S)-thiahomocitrate | |
1.1.1.87 | 2.97 | - |
recombinant His-tagged enzyme, pH 7.8, 20°C, competitive versus (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate | Candida albicans | (2R,3S)-3-(p-carboxybenzyl)malate | |
1.1.1.87 | 3.78 | - |
recombinant His-tagged enzyme, pH 7.8, 20°C | Candida albicans | (2R,3S)-3-(p-carboxybenzyl)malate |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.87 | additional information | three-dimensional structure and homology modeling, overview | Candida albicans |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.87 | 0.35 | - |
NAD+ | recombinant His-tagged enzyme, pH 7.8, 20°C | Candida albicans | |
1.1.1.87 | 0.44 | - |
NAD+ | native enzyme, pH 7.8, 20°C | Candida albicans | |
1.1.1.87 | 5.16 | - |
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate | recombinant His-tagged enzyme, pH 7.8, 20°C | Candida albicans | |
1.1.1.87 | 8.75 | - |
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate | native enzyme, pH 7.8, 20°C | Candida albicans |