Any feedback?
Literature summary extracted from
- Characterization of pyrolysin, a hyperthermoactive serine protease from the archaebacterium Pyrococcus furiosus (1990), FEMS Microbiol. Lett., 71, 17-20.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.B55 | diisopropyl fluorophosphate | 0.5 mM, complete inhibition | Pyrococcus furiosus |  |
| 3.4.21.B55 | EDTA | 10 mM, 27% inhibition | Pyrococcus furiosus | |
| 3.4.21.B55 | additional information | no inhibition by cysteine-type protease inhibitors pCMB and iodoacetate | Pyrococcus furiosus | |
| 3.4.21.B55 | PMSF | 1 mM, 87% inhibition | Pyrococcus furiosus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.B55 | cell envelope | 75% of the activity is associated with the cell-envelope | Pyrococcus furiosus | 30313 | - |
| 3.4.21.B55 | cytoplasm | 25% of the activity was located in the cytoplasm | Pyrococcus furiosus | 5737 | - |
| 3.4.21.B55 | additional information | no activity is observed in the medium when Pyrococcus furiosus is grown in batch culture | Pyrococcus furiosus | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.B55 | Pyrococcus furiosus | P72186 | - |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 3.4.21.B55 | oxygen-stable enzyme | Pyrococcus furiosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.B55 | casein + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.B55 | 115 | - |
- |
Pyrococcus furiosus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.B55 | 80 | - |
t1/2 is above 6000 min | Pyrococcus furiosus |
| 3.4.21.B55 | 95 | - |
t1/2: 540 min | Pyrococcus furiosus |
| 3.4.21.B55 | 100 | - |
t1/2: 240 min | Pyrococcus furiosus |
| 3.4.21.B55 | 105 | - |
t1/2: 20 min | Pyrococcus furiosus |
| 3.4.21.B55 | 110 | - |
t1/2: 3 min | Pyrococcus furiosus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.B55 | 6.5 | 10.5 | broad pH range with at least 50% of its maximal activity between pH 6.5 and 10.5 | Pyrococcus furiosus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
