BRENDA - Enzyme Database

Biochemical and molecular characterization of NAD(+)-dependent isocitrate dehydrogenase from the ethanologenic bacterium Zymomonas mobilis

Wang, P.; Jin, M.; Zhu, G.; FEMS Microbiol. Lett. 327, 134-141 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.41
gene idh, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3)
Zymomonas mobilis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
Ca2+
-
Zymomonas mobilis
1.1.1.41
Co2+
-
Zymomonas mobilis
1.1.1.41
Cu2+
complete inhibition
Zymomonas mobilis
1.1.1.41
Ni2+
-
Zymomonas mobilis
1.1.1.41
Zn2+
complete inhibition
Zymomonas mobilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.245
-
NAD+
pH 7.5, 37°C, with Mn2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
0.312
-
NAD+
pH 7.5, 37°C, with Mg2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
7.7
-
NADP+
pH 7.5, 37°C, with Mn2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
8.2
-
NADP+
pH 7.5, 37°C, with Mg2+, recombinant enzyme
Zymomonas mobilis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Co2+
activates, 42.5% activity compared to Mn2+
Zymomonas mobilis
1.1.1.41
Mg2+
activates, 78% activity compared to Mn2+
Zymomonas mobilis
1.1.1.41
Mn2+
activates, best cation
Zymomonas mobilis
1.1.1.41
additional information
the recombinant ZmIDH activity is completely dependent on the divalent cation and Mn2+ is the most effective cation
Zymomonas mobilis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.41
46000
-
2 * 46000, recombinant His6-tagged enzyme, SDS-PAGE
Zymomonas mobilis
1.1.1.41
74000
-
recombinant His6-tagged enzyme, gel filtration
Zymomonas mobilis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Zymomonas mobilis
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Zymomonas mobilis ATCC 10988
-
2-oxoglutarate + CO2 + NADH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Zymomonas mobilis
-
ssp. mobiliss
-
1.1.1.41
Zymomonas mobilis ATCC 10988
-
ssp. mobiliss
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.41
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3)
Zymomonas mobilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
722295
Zymomonas mobilis
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
poor performance of the recombinant ZmIDH in decarboxylation
722295
Zymomonas mobilis
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
722295
Zymomonas mobilis ATCC 10988
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
poor performance of the recombinant ZmIDH in decarboxylation
722295
Zymomonas mobilis ATCC 10988
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NADP+
poor performance of the recombinant ZmIDH in decarboxylation
722295
Zymomonas mobilis
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
poor performance of the recombinant ZmIDH in decarboxylation
722295
Zymomonas mobilis ATCC 10988
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.41
dimer
2 * 46000, recombinant His6-tagged enzyme, SDS-PAGE
Zymomonas mobilis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.41
55
-
-
Zymomonas mobilis
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.1.41
25
60
and above, activity range, profile overview
Zymomonas mobilis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.41
40
-
purified recombinant His-tagged enzyme, rapid inactivation above
Zymomonas mobilis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.41
14
-
NADP+
pH 7.5, 37°C, with Mg2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
25
-
NADP+
pH 7.5, 37°C, with Mn2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
88
-
NAD+
pH 7.5, 37°C, with Mg2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
112
-
NAD+
pH 7.5, 37°C, with Mn2+, recombinant enzyme
Zymomonas mobilis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
8
-
with Mn2+
Zymomonas mobilis
1.1.1.41
8.5
-
with Mg2+
Zymomonas mobilis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.41
6.5
10
activity range, profile overview
Zymomonas mobilis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
ZmIDH displays a 165fold (kcat/Km) preference for NAD+ over NADP+ with Mg2+, and 142fold with Mn2+
Zymomonas mobilis
1.1.1.41
NADP+
ZmIDH displays a 165fold (kcat/Km) preference for NAD+ over NADP+ with Mg2+, and 142fold with Mn2+
Zymomonas mobilis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
gene idh, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3)
Zymomonas mobilis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
ZmIDH displays a 165fold (kcat/Km) preference for NAD+ over NADP+ with Mg2+, and 142fold with Mn2+
Zymomonas mobilis
1.1.1.41
NADP+
ZmIDH displays a 165fold (kcat/Km) preference for NAD+ over NADP+ with Mg2+, and 142fold with Mn2+
Zymomonas mobilis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
Ca2+
-
Zymomonas mobilis
1.1.1.41
Co2+
-
Zymomonas mobilis
1.1.1.41
Cu2+
complete inhibition
Zymomonas mobilis
1.1.1.41
Ni2+
-
Zymomonas mobilis
1.1.1.41
Zn2+
complete inhibition
Zymomonas mobilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.245
-
NAD+
pH 7.5, 37°C, with Mn2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
0.312
-
NAD+
pH 7.5, 37°C, with Mg2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
7.7
-
NADP+
pH 7.5, 37°C, with Mn2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
8.2
-
NADP+
pH 7.5, 37°C, with Mg2+, recombinant enzyme
Zymomonas mobilis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Co2+
activates, 42.5% activity compared to Mn2+
Zymomonas mobilis
1.1.1.41
Mg2+
activates, 78% activity compared to Mn2+
Zymomonas mobilis
1.1.1.41
Mn2+
activates, best cation
Zymomonas mobilis
1.1.1.41
additional information
the recombinant ZmIDH activity is completely dependent on the divalent cation and Mn2+ is the most effective cation
Zymomonas mobilis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.41
46000
-
2 * 46000, recombinant His6-tagged enzyme, SDS-PAGE
Zymomonas mobilis
1.1.1.41
74000
-
recombinant His6-tagged enzyme, gel filtration
Zymomonas mobilis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Zymomonas mobilis
-
2-oxoglutarate + CO2 + NADH
-
-
?
1.1.1.41
isocitrate + NAD+
Zymomonas mobilis ATCC 10988
-
2-oxoglutarate + CO2 + NADH
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3)
Zymomonas mobilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
722295
Zymomonas mobilis
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
poor performance of the recombinant ZmIDH in decarboxylation
722295
Zymomonas mobilis
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
-
722295
Zymomonas mobilis ATCC 10988
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NAD+
poor performance of the recombinant ZmIDH in decarboxylation
722295
Zymomonas mobilis ATCC 10988
2-oxoglutarate + CO2 + NADH
-
-
-
?
1.1.1.41
isocitrate + NADP+
poor performance of the recombinant ZmIDH in decarboxylation
722295
Zymomonas mobilis
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.41
isocitrate + NADP+
poor performance of the recombinant ZmIDH in decarboxylation
722295
Zymomonas mobilis ATCC 10988
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.41
dimer
2 * 46000, recombinant His6-tagged enzyme, SDS-PAGE
Zymomonas mobilis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.41
55
-
-
Zymomonas mobilis
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.1.41
25
60
and above, activity range, profile overview
Zymomonas mobilis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.41
40
-
purified recombinant His-tagged enzyme, rapid inactivation above
Zymomonas mobilis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.41
14
-
NADP+
pH 7.5, 37°C, with Mg2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
25
-
NADP+
pH 7.5, 37°C, with Mn2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
88
-
NAD+
pH 7.5, 37°C, with Mg2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
112
-
NAD+
pH 7.5, 37°C, with Mn2+, recombinant enzyme
Zymomonas mobilis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
8
-
with Mn2+
Zymomonas mobilis
1.1.1.41
8.5
-
with Mg2+
Zymomonas mobilis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.41
6.5
10
activity range, profile overview
Zymomonas mobilis
General Information
EC Number
General Information
Commentary
Organism
1.1.1.41
evolution
the recombinant ZmIDH is mainly NAD+-dependent and its catalytic efficiency (kcat/Km) is relative low when compared to the prokaryotic NADP+-IDHs
Zymomonas mobilis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.41
evolution
the recombinant ZmIDH is mainly NAD+-dependent and its catalytic efficiency (kcat/Km) is relative low when compared to the prokaryotic NADP+-IDHs
Zymomonas mobilis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.000002
-
NADP+
pH 7.5, 37°C, with Mg2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
0.000003
-
NADP+
pH 7.5, 37°C, with Mn2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
0.0003
-
NAD+
pH 7.5, 37°C, with Mg2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
0.0005
-
NAD+
pH 7.5, 37°C, with Mn2+, recombinant enzyme
Zymomonas mobilis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.000002
-
NADP+
pH 7.5, 37°C, with Mg2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
0.000003
-
NADP+
pH 7.5, 37°C, with Mn2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
0.0003
-
NAD+
pH 7.5, 37°C, with Mg2+, recombinant enzyme
Zymomonas mobilis
1.1.1.41
0.0005
-
NAD+
pH 7.5, 37°C, with Mn2+, recombinant enzyme
Zymomonas mobilis