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Literature summary extracted from

  • Carrizo, M.; Romero, J.; Issoglio, F.; Curtino, J.
    Structural and biochemical insight into glycogenin inactivation by the glycogenosis-causing T82M mutation (2012), FEBS Lett., 586, 254-257.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.186 expression of wild-type gycogenin-1, its truncated mutant DELTA-270, and of glycogenin-1 point mutants in Escherichia coli strain CGSC 4997 Oryctolagus cuniculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.186 purified recombinant glycogenin-1 truncation mutant DELTA-270, hanging drop vapor diffusion method, the protein in 12% w/v, PEG monomethyl ester 5000, 0.1 M MES, pH 6.5, and 0.2 M ammonium sulfate, is mixed with 1 M ammonium sulfate and 0.1 M sodium phosphate, pH 6.7, 4°C, crystals are soaked in mother liquor containing 1 mM MnSO4 and 1 mM UDP or UDP-glucose, X-ray diffraction structure determination and analysis Oryctolagus cuniculus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.186 T82M inactive mutant, the mutation is equivalent to T83M according to previous authors amino acid numbering, it causes glycogenosis showing the loss of Thr82 hydrogen bond to Asp162, the residue involved in the activation step of the glucose transfer reaction mechanism. Autoglucosylation, maltoside transglucosylation and UDP-glucose hydrolyzing activities are abolished Oryctolagus cuniculus
2.4.1.186 T83S site-directed mutagenesis, the mutant is catalytically active Oryctolagus cuniculus
2.4.1.186 T83V site-directed mutagenesis, inactive mutant Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.186 Mn2+ required Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.186 UDP-alpha-D-glucose + glycogenin Oryctolagus cuniculus autoglucosylation by glycogenin-1 UDP + alpha-D-glucosylglycogenin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.186 Oryctolagus cuniculus P13280
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.186 recombinant wild-type gycogenin-1, its truncated mutant DELTA-270, and of glycogenin-1 point mutants from Escherichia coli strain CGSC 4997 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.186 UDP-alpha-D-glucose + glycogenin autoglucosylation by glycogenin-1 Oryctolagus cuniculus UDP + alpha-D-glucosylglycogenin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.186 glycogenin-1
-
 Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.186 30
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.186 7
-
 assay at Oryctolagus cuniculus

General Information

EC Number General Information Comment Organism
2.4.1.186 malfunction the glycogenin-1 mutation T82M causes glycogenosis. Substitution of Thr82 for serine but not for valine restores the maximum extent of autoglucosylation as well as transglucosylation and UDP-glucose hydrolysis rate, structure analysis, overview Oryctolagus cuniculus
2.4.1.186 physiological function Asp162 is the residue involved in the activation step of the glucose transfer reaction mechanism Oryctolagus cuniculus