Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Guranowski, A.; Wojdyla, A.; Zimny, J.; Wypijewska, A.; Kowalska, J.; Jemielity, J.; Davis, R.; Bieganowski, P.
    Dual activity of certain HIT-proteins: A. thaliana Hint4 and C. elegans DcpS act on adenosine 5'-phosphosulfate as hydrolases (forming AMP) and as phosphorylases (forming ADP) (2010), FEBS Lett., 584, 93-98.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.5 1
-
 adenosine 5'-phosphosulfate in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C Arabidopsis thaliana
2.7.7.5 26
-
 adenosine 5'-phosphosulfate in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C Caenorhabditis elegans
2.7.7.5 46
-
 phosphate in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C Caenorhabditis elegans
2.7.7.5 600
-
 phosphate in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.5 Arabidopsis thaliana Q84VV6
-
-
2.7.7.5 Caenorhabditis elegans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.5 additional information the enzyme can act as a sulfohydrolase, catalyzing the liberation of AMP from adenosine 5'-phosphosulfate. However, in the presence of phosphate, the enzyme also behaves as adenosine 5'-phosphosulfate phosphorylases, forming ADP Arabidopsis thaliana ?
-
 ?
2.7.7.5 additional information the enzyme can act as a sulfohydrolase, catalyzing the liberation of AMP from adenosine 5'-phosphosulfate. However, in the presence of phosphate, the enzyme also behaves as adenosine 5'-phosphosulfate phosphorylases, forming ADP. The enzyme does not use adenosine 5'-phosphoamidate, adenosine 5'-phosphofluoride, and diadenosine tetraphosphate as substrates Caenorhabditis elegans ?
-
 ?
2.7.7.5 additional information when assayed in 0.1 M KH2PO4 with adenosine 5'-phosphosulfate, the enzyme cannot use diphosphate or triphosphate as potential adenylate acceptors in place of phosphate Arabidopsis thaliana ?
-
 ?
2.7.7.5 additional information when assayed in 0.1 M KH2PO4 with adenosine 5'-phosphosulfate, the enzyme cannot use diphosphate or tripolyphosphate as potential adenylate acceptors in place of phosphate Caenorhabditis elegans ?
-
 ?
2.7.7.5 phosphate + adenosine 5'-phosphoamidate 22% reaction velocity compared to adenosine 5'-phosphosulfate Arabidopsis thaliana ADP + NH4+
-
 ir
2.7.7.5 phosphate + adenosine 5'-phosphofluoride 8% reaction velocity compared to adenosine 5'-phosphosulfate Arabidopsis thaliana ADP + fluoride
-
 ?
2.7.7.5 phosphate + adenosine 5'-phosphosulfate 100% reaction velocity Caenorhabditis elegans ADP + SO42-
-
 ir
2.7.7.5 phosphate + adenosine 5'-phosphosulfate 100% reaction velocity Arabidopsis thaliana ADP + SO42-
-
 ir
2.7.7.5 phosphate + diadenosine tetraphosphate 2% reaction velocity compared to adenosine 5'-phosphosulfate Arabidopsis thaliana ?
-
 ?
2.7.7.5 phosphate + diadenosine triphosphate 4% reaction velocity compared to adenosine 5'-phosphosulfate Caenorhabditis elegans ?
-
 ?
2.7.7.5 phosphate + diadenosine triphosphate 7% reaction velocity compared to adenosine 5'-phosphosulfate Arabidopsis thaliana ?
-
 ?
2.7.7.5 phosphate + inosine 5'-phosphosulfate
-
 Caenorhabditis elegans IDP + SO42-
-
 ir
2.7.7.5 phosphate + inosine 5'-phosphosulfate
-
 Arabidopsis thaliana IDP + SO42-
-
 ir

Subunits

EC Number Subunits Comment Organism
2.7.7.5 homodimer
-
 Caenorhabditis elegans
2.7.7.5 homodimer
-
 Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.7.5 adenosine 5'-phosphosulfate phosphorylase
-
 Caenorhabditis elegans
2.7.7.5 adenosine 5'-phosphosulfate phosphorylase
-
 Arabidopsis thaliana
2.7.7.5 adenylylsulfate:phosphate adenylyltransferase
-
 Caenorhabditis elegans
2.7.7.5 adenylylsulfate:phosphate adenylyltransferase
-
 Arabidopsis thaliana
2.7.7.5 ADP-sulfurylase misleading Caenorhabditis elegans
2.7.7.5 ADP-sulfurylase misleading Arabidopsis thaliana
2.7.7.5 APS phosphorylase
-
 Caenorhabditis elegans
2.7.7.5 APS phosphorylase
-
 Arabidopsis thaliana
2.7.7.5 Dcps
-
 Caenorhabditis elegans
2.7.7.5 Hint4
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.5 2.6
-
 adenosine 5'-phosphosulfate in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C Caenorhabditis elegans
2.7.7.5 3 6 adenosine 5'-phosphosulfate in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.5 4.2 6.8 Hint4 degrades adenosine 5'-phosphosulfate phosphorolytically twice as fast at pH 4.2 than at pH 6.8 Arabidopsis thaliana