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Literature summary extracted from

  • Graindorge, M.; Giustini, C.; Jacomin, A.C.; Kraut, A.; Curien, G.; Matringe, M.
    Identification of a plant gene encoding glutamate/aspartate-prephenate aminotransferase: the last homeless enzyme of aromatic amino acids biosynthesis (2010), FEBS Lett., 584, 4357-4360.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.78 expression in Escherichia coli Arabidopsis thaliana
2.6.1.79 expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.78 0.025
-
oxaloacetate pH 8.0, 30°C Arabidopsis thaliana
2.6.1.78 12
-
L-aspartate pH 8.0, 30°C Arabidopsis thaliana
2.6.1.79 0.2
-
2-oxoglutarate pH 8.0, 30°C Arabidopsis thaliana
2.6.1.79 5.6
-
L-glutamate pH 8.0, 30°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.78 plastid
-
Arabidopsis thaliana 9536
-
2.6.1.79 plastid
-
Arabidopsis thaliana 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.78 43000
-
x * 43000, SDS-PAGE Arabidopsis thaliana
2.6.1.79 43000
-
x * 43000, SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.78 Arabidopsis thaliana Q9SIE1 bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase
-
2.6.1.79 Arabidopsis thaliana Q9SIE1 bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.78 28.2
-
pH 8.0, 30°C Arabidopsis thaliana
2.6.1.79 28.2
-
pH 8.0, 30°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.78 L-arogenate + oxaloacetate
-
Arabidopsis thaliana prephenate + L-aspartate
-
r
2.6.1.78 additional information no substrates: 4-hydroxyphenylpyruvate, phenylpyruvate Arabidopsis thaliana ?
-
?
2.6.1.78 prephenate + L-aspartate
-
Arabidopsis thaliana L-arogenate + oxaloacetate
-
r
2.6.1.79 L-arogenate + 2-oxoglutarate
-
Arabidopsis thaliana prephenate + L-glutamate
-
r
2.6.1.79 additional information no substrates: 4-hydroxyphenylpyruvate, phenylpyruvate Arabidopsis thaliana ?
-
?
2.6.1.79 prephenate + L-glutamate
-
Arabidopsis thaliana L-arogenate + 2-oxoglutarate
-
r

Subunits

EC Number Subunits Comment Organism
2.6.1.78 ? x * 43000, SDS-PAGE Arabidopsis thaliana
2.6.1.79 ? x * 43000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.6.1.78 Pat
-
Arabidopsis thaliana
2.6.1.78 prephenate aminotransferase
-
Arabidopsis thaliana
2.6.1.79 Pat
-
Arabidopsis thaliana
2.6.1.79 prephenate aminotransferase
-
Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.78 65
-
L-aspartate pH 8.0, 30°C Arabidopsis thaliana
2.6.1.79 200
-
L-glutamate pH 8.0, 30°C Arabidopsis thaliana