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Literature summary extracted from

  • Drewke, C., Klein, M.; Clade, D.; Arenz, A.; Muller, R.; Leistner, E.
    4-O-phosphoryl-L-threonine, a substrate of the pdxC(serC) gene product involved in vitamin B6 biosynthesis (1996), FEBS Lett., 390, 179-82.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.52 0.01
-
 3-phosphonooxypyruvate MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C Escherichia coli
2.6.1.52 0.015
-
 3-phosphonooxypyruvate PdxC(SerC) protein, pH 8.2, 37°C Escherichia coli
2.6.1.52 0.017
-
 3-O-phospho-L-serine PdxC(SerC) protein, pH 8.2, 37°C Escherichia coli
2.6.1.52 0.037
-
 3-O-phospho-L-serine MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C Escherichia coli
2.6.1.52 0.11
-
 4-(phosphonooxy)-L-threonine MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C Escherichia coli
2.6.1.52 0.11
-
 4-(phosphonooxy)-L-threonine PdxC(SerC) protein, pH 8.2, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.52 36000
-
 PdxC(SerC) protein Escherichia coli
2.6.1.52 79000
-
 MalE/PdxC(SerC) fusion protein, Coomassie blue stained SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.52 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.52 MalE/PdxC(SerC) fusion protein, affinity chromatography on an amylose resin, hydrolyzed in the presence of protease factor Xa Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.6.1.52 Whereas the fusion protein is stable over several weeks, the cleaved protein shows a clear loss of activity within 2 weeks at 4°C. Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.52 2-oxo-3-hydroxy-4-phosphobutanoate + L-glutamate 2-oxo-3-hydroxy-4-phosphobutanoate = 3-hydroxy-4-O-phosphoryl-2-oxobutyrate Escherichia coli 4-(phosphonooxy)-L-threonine + 2-oxoglutarate 4-phospho-hydroxy-L-threonine = 4-O-phosphoryl-L-threonine ?
2.6.1.52 3-O-phospho-L-serine + 2-oxoglutarate
-
 Escherichia coli ?
-
 ?
2.6.1.52 3-phosphonooxypyruvate + 2-oxoglutarate
-
 Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.52 3-O-phospho-L-serine:2-oxoglutarate aminotransferase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.52 0.08
-
 4-(phosphonooxy)-L-threonine MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C Escherichia coli
2.6.1.52 0.15
-
 4-(phosphonooxy)-L-threonine PdxC(SerC) protein, pH 8.2, 37°C Escherichia coli
2.6.1.52 0.39
-
 3-O-phospho-L-serine PdxC(SerC) protein, pH 8.2, 37°C Escherichia coli
2.6.1.52 0.63
-
 3-O-phospho-L-serine MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C Escherichia coli
2.6.1.52 1.33
-
 3-phosphonooxypyruvate MalE/PdxC(SerC) fusion protein, pH 8.2, 37°C Escherichia coli
2.6.1.52 1.75
-
 3-phosphonooxypyruvate PdxC(SerC) protein, pH 8.2, 37°C Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.52 7.8 8.8
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.52 pyridoxal 5'-phosphate
-
 Escherichia coli