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Literature summary extracted from

  • Gus-Mayer, S.; Brunner, H.; Schneider-Poetsch, H.A.; Lottspeich, F.; Eckerskorn, C.; Grimm, R.; Rüdiger, W.
    The amino acid sequence previously attributed to a protein kinase or a TCP1-related molecular chaperone and co-purified with phytochrome is a beta-glucosidase (1994), FEBS Lett., 347, 51-54.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.186
-
 Avena sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.188 60000
-
 x * 60000, SDS-PAGE Avena sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.186 avenacoside + H2O Avena sativa the enzyme is part of the defense system of Arena sativa. Avenacosides are hydrolyzed to 26-desgluco-avenacosides immediately after damage of the plant tissue. Only the desgluco-derivatives exhibit fungicidal activity 26-deglucoavenacoside + D-glucose
-
 ?
3.2.1.188 avenacoside B + H2O Avena sativa
-
 26-desgluco-avenacoside B + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.186 Avena sativa Q38786
-
-
3.2.1.188 Avena sativa
-
 cultivar Pirol
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.188 hydroxyapatite column chromatography Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.186 seedling etiolated Avena sativa
-
3.2.1.188 seedling
-
 Avena sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.188 1.73
-
 using 4-nitrophenyl-beta-galactoside as substrate, at pH 5.4 and 34°C for 15 min Avena sativa
3.2.1.188 2.72
-
 using 4-nitrophenyl-beta-glucoside as substrate, at pH 5.4 and 34°C for 15 min Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.186 4-nitrophenyl beta-D-glucoside + H2O
-
 Avena sativa 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.186 avenacoside + H2O the enzyme is part of the defense system of Arena sativa. Avenacosides are hydrolyzed to 26-desgluco-avenacosides immediately after damage of the plant tissue. Only the desgluco-derivatives exhibit fungicidal activity Avena sativa 26-deglucoavenacoside + D-glucose
-
 ?
3.2.1.188 4-nitrophenyl beta-D-galactoside + H2O
-
 Avena sativa 4-nitrophenol + D-galactopyranose
-
 ?
3.2.1.188 4-nitrophenyl beta-D-glucoside + H2O
-
 Avena sativa 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.188 avenacoside B + H2O
-
 Avena sativa 26-desgluco-avenacoside B + D-glucose
-
 ?
3.2.1.188 additional information no activity with 4-nitrophenyl-alpha-glucoside and 4-nitrophenyl-alpha-galactoside Avena sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.188 ? x * 60000, SDS-PAGE Avena sativa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.186 avenacosidase
-
 Avena sativa