Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Clausen, A.R.; Girandon, L.; Ali, A.; Knecht, W.; Rozpedowska, E.; Sandrini, M.P.; Andreasson, E.; Munch-Petersen, B.; Piskur, J.
    Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana (2012), FEBS J., 279, 3889-3897.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21 expressed in Escherichia coli KY895 cells Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.00038
-
 3'-azido-3'-deoxythymidine isoform TK1b, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 0.00062
-
 thymidine isoform TK1a, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 0.0014
-
 thymidine isoform TK1b, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 0.0027
-
 3'-azido-3'-deoxythymidine isoform TK1a, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 0.0066
-
 deoxyuridine isoform TK1b, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 0.015
-
 deoxyuridine isoform TK1a, pH and temperature not specified in the publication Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.21 26100
-
 x * 26100, isoform TK1a, calculated from amino acid sequence Arabidopsis thaliana
2.7.1.21 30700
-
 x * 30700, isoform TK1b, calculated from amino acid sequence Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Arabidopsis thaliana Q8LDP6 ecotype Columbia-0
-
2.7.1.21 Arabidopsis thaliana Q9FN47 ecotype Columbia-0
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21
-
 Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.21 bud
-
 Arabidopsis thaliana
-
2.7.1.21 silique
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + 3'-azido-3'-deoxythymidine
-
 Arabidopsis thaliana ADP + 3'-azido-3'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.21 ATP + deoxyuridine
-
 Arabidopsis thaliana ADP + dUMP
-
 ?
2.7.1.21 ATP + thymidine best substrate Arabidopsis thaliana ADP + dTMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.21 ? x * 26100, isoform TK1a, calculated from amino acid sequence Arabidopsis thaliana
2.7.1.21 ? x * 30700, isoform TK1b, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.1.21 thymidine kinase 1
-
 Arabidopsis thaliana
2.7.1.21 TK1
-
 Arabidopsis thaliana
2.7.1.21 TK1a isoform Arabidopsis thaliana
2.7.1.21 TK1b isoform Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.21 0.009
-
 3'-azido-3'-deoxythymidine isoform TK1b, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 0.02
-
 thymidine isoform TK1b, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 0.045
-
 deoxyuridine isoform TK1b, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 0.13
-
 3'-azido-3'-deoxythymidine isoform TK1a, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 0.48
-
 thymidine isoform TK1a, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 1.5
-
 deoxyuridine isoform TK1a, pH and temperature not specified in the publication Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.7.1.21 malfunction TK1a/TK1b double mutants die at an early stage Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.21 6.8
-
 deoxyuridine isoform TK1b, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 14
-
 thymidine isoform TK1b, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 22
-
 3'-azido-3'-deoxythymidine isoform TK1b, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 48
-
 3'-azido-3'-deoxythymidine isoform TK1a, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 150
-
 deoxyuridine isoform TK1a, pH and temperature not specified in the publication Arabidopsis thaliana
2.7.1.21 770
-
 thymidine isoform TK1a, pH and temperature not specified in the publication Arabidopsis thaliana