EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.21 | expressed in Escherichia coli BL21 cells | Caenorhabditis elegans |
2.7.1.21 | expressed in Escherichia coli BL21 cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.1.21 | mutant enzyme T163S, hanging drop vapor diffusion method, using 0.1 M sodium citrate (pH 5.6), 20% (v/v) 2-propanol and 17% (w/v) polyethylene glycol 4000 | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.21 | L124A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
2.7.1.21 | L143A | the mutant shows reduced catalytic efficiency with thymidine compared to the wild type enzyme and exhibits activity towards deoxyguanosine | Caenorhabditis elegans |
2.7.1.21 | M28A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Homo sapiens |
2.7.1.21 | M28I | the mutation has a negative impact on enzyme activity with thymidine as the substrate, affecting both the Km (250fold increase) and the kcat (6fold increase) values. The mutant has no activity with deoxycytidine | Homo sapiens |
2.7.1.21 | M42I | the mutation has a negative impact on enzyme activity with thymidine as the substrate, affecting both the Km (250fold increase) and the kcat (2fold increase) values | Caenorhabditis elegans |
2.7.1.21 | S182A | the mutant shows reduced catalytic efficiency with thymidine compared to the wild type enzyme and exhibits activity towards deoxyguanosine | Caenorhabditis elegans |
2.7.1.21 | S182T | the mutant shows reduced catalytic efficiency with thymidine compared to the wild type enzyme and exhibits activity towards deoxyguanosine | Caenorhabditis elegans |
2.7.1.21 | T163S | the mutation results in a kinase with a 140fold lower Km for 3-azido-3-deoxythymidine | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.21 | 0.00043 | - |
3'-azido-3'-deoxythymidine | mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.00052 | - |
3'-azido-3'-deoxythymidine | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.0013 | - |
thymidine | mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.0014 | - |
thymidine | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.0023 | - |
thymidine | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.0036 | - |
deoxycytidine | mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.012 | - |
3'-azido-3'-deoxythymidine | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.018 | - |
3'-azido-3'-deoxythymidine | mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.033 | - |
3'-azido-3'-deoxythymidine | mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.036 | - |
thymidine | mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.059 | - |
thymidine | mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.074 | - |
thymidine | mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.204 | - |
3'-azido-3'-deoxythymidine | mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.254 | - |
3'-azido-3'-deoxythymidine | mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.317 | - |
thymidine | mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.336 | - |
thymidine | mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.349 | - |
thymidine | mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.406 | - |
3'-azido-3'-deoxythymidine | mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.565 | - |
thymidine | mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 2.318 | - |
3'-azido-3'-deoxythymidine | mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.21 | Caenorhabditis elegans | - |
- |
- |
2.7.1.21 | Homo sapiens | P04183 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.21 | GST affinity column chromatography | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.21 | ATP + 3'-azido-3'-deoxythymidine | - |
Caenorhabditis elegans | ADP + 3'-azido-3'-deoxythymidine 5'-phosphate | - |
? | |
2.7.1.21 | ATP + 3'-azido-3'-deoxythymidine | - |
Homo sapiens | ADP + 3'-azido-3'-deoxythymidine 5'-phosphate | - |
? | |
2.7.1.21 | ATP + deoxycytidine | substrate for mutant enzymes M42I and L134A | Caenorhabditis elegans | ADP + dCMP | - |
? | |
2.7.1.21 | ATP + deoxyguanosine | substrate for mutant enzymes S182T and S182A | Caenorhabditis elegans | ADP + dGMP | - |
? | |
2.7.1.21 | ATP + thymidine | - |
Caenorhabditis elegans | ADP + dTMP | - |
? | |
2.7.1.21 | ATP + thymidine | - |
Homo sapiens | ADP + dTMP | - |
? | |
2.7.1.21 | additional information | the wild type enzyme shows no activity with deoxycytidine and deoxyadenosine | Caenorhabditis elegans | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.21 | thymidine kinase 1 | - |
Caenorhabditis elegans |
2.7.1.21 | thymidine kinase 1 | - |
Homo sapiens |
2.7.1.21 | TK1 | - |
Caenorhabditis elegans |
2.7.1.21 | TK1 | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.21 | 0.17 | - |
deoxycytidine | mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.24 | - |
3'-azido-3'-deoxythymidine | mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.34 | - |
3'-azido-3'-deoxythymidine | mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.58 | - |
3'-azido-3'-deoxythymidine | mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.74 | - |
thymidine | mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.81 | - |
3'-azido-3'-deoxythymidine | mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.97 | - |
thymidine | mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 1.3 | - |
3'-azido-3'-deoxythymidine | mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 1.4 | - |
3'-azido-3'-deoxythymidine | mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 1.4 | - |
thymidine | mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 1.5 | - |
thymidine | mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 1.5 | - |
3'-azido-3'-deoxythymidine | mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 1.8 | - |
thymidine | mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 2.7 | - |
thymidine | mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 2.8 | - |
thymidine | mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 3.5 | - |
3'-azido-3'-deoxythymidine | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 4.9 | - |
thymidine | mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 6.4 | - |
thymidine | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 9.3 | - |
3'-azido-3'-deoxythymidine | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 9.5 | - |
thymidine | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.21 | 0.0019 | - |
deoxycytidine | mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.0028 | - |
thymidine | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.0031 | - |
3'-azido-3'-deoxythymidine | mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.0066 | - |
deoxyguanosine | mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.0067 | - |
3'-azido-3'-deoxythymidine | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.0068 | - |
thymidine | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.048 | - |
deoxycytidine | mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.078 | - |
3'-azido-3'-deoxythymidine | wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.23 | - |
deoxyguanosine | mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.35 | - |
3'-azido-3'-deoxythymidine | mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.44 | - |
3'-azido-3'-deoxythymidine | mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.46 | - |
thymidine | mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.49 | - |
3'-azido-3'-deoxythymidine | mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 0.51 | - |
thymidine | mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 0.94 | - |
3'-azido-3'-deoxythymidine | mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 1.4 | - |
3'-azido-3'-deoxythymidine | mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 1.7 | - |
3'-azido-3'-deoxythymidine | mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 4.2 | - |
thymidine | mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 4.5 | - |
thymidine | mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Homo sapiens | |
2.7.1.21 | 5 | - |
thymidine | mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 10 | - |
thymidine | mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 15 | - |
thymidine | mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 98 | - |
3'-azido-3'-deoxythymidine | mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans | |
2.7.1.21 | 750 | - |
thymidine | mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication | Caenorhabditis elegans |