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Literature summary extracted from

  • Torchetti, E.; Bonomi, F.; Galluccio, M.; Gianazza, E.; Giancaspero, T.; Iametti, S.; Indiveri, C.; Barile, M.
    Human FAD synthase (isoform 2): A component of the machinery that delivers FAD to apo-flavoproteins (2011), FEBS J., 278, 4435-4449.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.2 His6-tagged enzyme is expressed in Escherichia coli Rosetta (DE3) cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.2 GDP competitive inhibition Homo sapiens
2.7.7.2 GTP uncompetitive inhibition Homo sapiens
2.7.7.2 Urea the enzymatic activity of isoform FADS2 decreases dramatically at increasing urea concentration, with a mid-point for activity loss at 2.1 m urea Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.2 0.00035
-
 FMN at 37°C, in 50 mM Tris-HCl, pH 7.5 Homo sapiens
2.7.7.2 0.0153
-
 ATP at 37°C, in 50 mM Tris-HCl, pH 7.5 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.2 cytosol
-
 Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.2 Ca2+ Ca2+ gives maximum FAD synthesis equal to 62% of that measured with Mg2+ Homo sapiens
2.7.7.2 Co2+ in the presence of about 0.5 mM Co2+, the activity is almost equal to that measured with Mg2+ Homo sapiens
2.7.7.2 Mg2+ MgCl2 is strictly required for both the synthetic and diphosphorolytic activity, FAD synthesis is abolished in the absence of MgCl2 Homo sapiens
2.7.7.2 Mn2+ Mn2+ gives maximum FAD synthesis equal to 77% of that measured with Mg2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.2 56537
-
 x * 56537, isoform FADS2, calculated from amino acid sequence Homo sapiens
2.7.7.2 57000
-
 x * 57000, isoform FADS2, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.2 Homo sapiens Q8NFF5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.2 Ni2+-chelating column chromatography and hydroxyapatite column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.2 ATP + FMN
-
 Homo sapiens diphosphate + FAD
-
 r
2.7.7.2 diphosphate + FAD low reaction rate Homo sapiens ATP + FMN
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.2 ? x * 56537, isoform FADS2, calculated from amino acid sequence Homo sapiens
2.7.7.2 ? x * 57000, isoform FADS2, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.7.2 FADS2 isoform Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.2 60
-
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.2 0.078
-
 FMN at 37°C, in 50 mM Tris-HCl, pH 7.5 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.2 7
-
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.2 1.8
-
 GTP at 37°C, in 50 mM Tris-HCl, pH 7.5 Homo sapiens
2.7.7.2 2.5
-
 GDP at 37°C, in 50 mM Tris-HCl, pH 7.5 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.2 Homo sapiens isoelectric focusing
-
 5.66
2.7.7.2 Homo sapiens calculated from amino acid sequence
-
 6