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Literature summary extracted from

  • Wu, H.J.; Yang, Y.; Wang, S.; Qiao, J.Q.; Xia, Y.F.; Wang, Y.; Wang, W.D.; Gao, S.F.; Liu, J.; Xue, P.Q.; Gao, X.W.
    Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 (2011), FEBS J., 278, 1345-1357.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.1 expressed in Escherichia coli as a His-tagged fusion protein Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.1 D232N mutant shows no activity Bacillus subtilis
2.6.1.1 K270H mutant shows no activity Bacillus subtilis
2.6.1.1 R403Y mutant shows no activity Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.1 0.3
-
 2-oxoglutarate pH 8.0, 45°C Bacillus subtilis
2.6.1.1 0.6
-
 oxaloacetate pH 8.0, 45°C Bacillus subtilis
2.6.1.1 6.7
-
 L-aspartate pH 8.0, 45°C Bacillus subtilis
2.6.1.1 8
-
 L-glutamate pH 8.0, 45°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.6.1.1 CaCl2 1 mM relative activity: 115.5% Bacillus subtilis
2.6.1.1 CoCl2 1 mM relative activity: 122.1% Bacillus subtilis
2.6.1.1 CuSO4 1 mM relative activity: 84.3% Bacillus subtilis
2.6.1.1 EDTA 1 mM relative activity: 106.4% Bacillus subtilis
2.6.1.1 MgCl2 1 mM relative activity: 96.9% Bacillus subtilis
2.6.1.1 MnSO4 1 mM relative activity: 90.1% Bacillus subtilis
2.6.1.1 ZnSO4 1 mM relative activity: 99.7% Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.1 49100
-
 calculated from cDNA Bacillus subtilis
2.6.1.1 55000
-
 SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.1 Bacillus subtilis Q939C9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.1 L-aspartate + 2-oxoglutarate relative activity L-aspartate: 100% Bacillus subtilis oxaloacetate + L-glutamate
-
 ?
2.6.1.1 L-aspartate + oxaloacetate relative activity compared to 2-oxoglutarate: 81.5% Bacillus subtilis ?
-
 ?
2.6.1.1 L-glutamate + 2-oxoglutarate relative activity compared to L-aspartate: 46.7% Bacillus subtilis 2-oxoglutarate + L-glutamate
-
 ?
2.6.1.1 L-phenylalanine + 2-oxoglutarate relative activity compared to L-aspartate: 0.3% Bacillus subtilis 2-oxo-3-phenylpropionic acid + L-glutamate
-
 ?
2.6.1.1 L-tryptophan + 2-oxoglutarate relative activity compared to L-aspartate: 1.7% Bacillus subtilis 3-indole-2-oxopropionic acid + L-glutamate
-
 ?
2.6.1.1 L-tyrosine + 2-oxoglutarate relative activity compared to L-aspartate: 0.4 Bacillus subtilis 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.1 monomer
-
 Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.6.1.1 aatB3
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.1 45
-
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.1 50
-
 enzyme stable up to 50°C Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.1 14
-
 L-glutamate pH 8.0, 45°C Bacillus subtilis
2.6.1.1 22
-
 oxaloacetate pH 8.0, 45°C Bacillus subtilis
2.6.1.1 27
-
 2-oxoglutarate pH 8.0, 45°C Bacillus subtilis
2.6.1.1 30
-
 L-aspartate pH 8.0, 45°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.1 8
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.1 7 9 relatively high activity in alkaline range Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.1 pyridoxal 5'-phosphate
-
 Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.1 1.75
-
 L-glutamate pH 8.0, 45°C Bacillus subtilis
2.6.1.1 4.5
-
 L-aspartate pH 8.0, 45°C Bacillus subtilis
2.6.1.1 36.67
-
 oxaloacetate pH 8.0, 45°C Bacillus subtilis
2.6.1.1 84.38
-
 2-oxoglutarate pH 8.0, 45°C Bacillus subtilis