EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.43 | additional information | no necessity of including a sulfhydryl reagent, such as dithiothreitol, in the assay reaction | Neisseria meningitidis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.43 | D209A | almost complete loss of activity. Residue involved in metal binding | Neisseria meningitidis |
2.7.7.43 | D211A | dramatic loss of activity. Residue involved in metal binding | Neisseria meningitidis |
2.7.7.43 | F192A | 8fold increase on Km value for CTP | Neisseria meningitidis |
2.7.7.43 | F193A | 3fold increase on Km value for CTP | Neisseria meningitidis |
2.7.7.43 | K142A | 10000fold reduction in kcat with an insignificant, fourfold, rise in Km for CTP | Neisseria meningitidis |
2.7.7.43 | K142A | dramatic loss of activity. Residue involved in metal binding | Neisseria meningitidis |
2.7.7.43 | N175A | 16fold increases in the Km value for CTP and 23fold for N-acetylneuraminate | Neisseria meningitidis |
2.7.7.43 | Q104A | 40fold inccrease in Km value for N-acetylneuraminate | Neisseria meningitidis |
2.7.7.43 | Q104E | dramatic loss of activity. Residue involved in metal binding | Neisseria meningitidis |
2.7.7.43 | Q104L | dramatic loss of activity. Residue involved in metal binding | Neisseria meningitidis |
2.7.7.43 | Q104N | dramatic loss of activity. Residue involved in metal binding | Neisseria meningitidis |
2.7.7.43 | R173A | 38fold increase in Km value for N-acetylneuraminate | Neisseria meningitidis |
2.7.7.43 | Y179A | 200fold decrease in kcat value | Neisseria meningitidis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.43 | 0.017 | - |
CTP | wild-type, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.026 | - |
CTP | mutant D211A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.031 | - |
CTP | mutant Q104A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.04 | - |
CTP | mutant Y179A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.048 | - |
CTP | mutant R173A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.052 | - |
CTP | mutant F193A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.066 | - |
N-acetylneuraminate | mutant D211A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.068 | - |
N-acetylneuraminate | wild-type, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.11 | - |
N-acetylneuraminate | mutant Y179A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.15 | - |
CTP | mutant F192A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.27 | - |
CTP | mutant N175A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.29 | - |
N-acetylneuraminate | mutant R173A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.38 | - |
N-acetylneuraminate | mutant F192A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.78 | - |
N-acetylneuraminate | mutant F193A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 1.6 | - |
N-acetylneuraminate | mutant N175A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 2.6 | - |
N-acetylneuraminate | mutant Q104A, pH 7.5, 22°C | Neisseria meningitidis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.43 | Mg2+ | enzyme recruits two Mg2+ ions during the catalytic cycle | Neisseria meningitidis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.43 | Neisseria meningitidis | P0A0Z8 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.7.43 | CTP + N-acetylneuraminate = diphosphate + CMP-N-acylneuraminate | typical ordered-sequential kinetic behaviour, enzyme does not bind N-acetylneuraminate in the absence of CTP | Neisseria meningitidis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.43 | CTP + N-acetylneuraminate | - |
Neisseria meningitidis | diphosphate + CMP-N-acetylneuraminate | - |
? | |
2.7.7.43 | additional information | no substrate: 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid, kcat/Km value is about 5000fold lower than that of N-acetylneuraminate | Neisseria meningitidis | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.43 | CMP-Neu5Ac synthetase | - |
Neisseria meningitidis |
2.7.7.43 | CNS | - |
Neisseria meningitidis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.43 | 0.001 | - |
CTP | mutant D211A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.05 | - |
CTP | mutant Y179A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 2.2 | - |
CTP | mutant Q104A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 5 | - |
CTP | mutant F193A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 7 | - |
CTP | mutant R173A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 10.2 | - |
CTP | wild-type, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 20 | - |
CTP | mutant F192A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 25 | - |
CTP | mutant N175A, pH 7.5, 22°C | Neisseria meningitidis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.43 | 0.0005 | - |
N-acetylneuraminate | mutant Y179A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.0009 | - |
N-acetylneuraminate | mutant Q104A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.0013 | - |
CTP | mutant Y179A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.0063 | - |
N-acetylneuraminate | mutant F193A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.052 | - |
N-acetylneuraminate | mutant F192A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.072 | - |
CTP | mutant Q104A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.09 | - |
CTP | mutant N175A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.097 | - |
CTP | mutant F193A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.132 | - |
CTP | mutant F192A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.143 | - |
CTP | mutant R173A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.148 | - |
N-acetylneuraminate | wild-type, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.155 | - |
N-acetylneuraminate | mutant N175A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.23 | - |
N-acetylneuraminate | mutant R173A, pH 7.5, 22°C | Neisseria meningitidis | |
2.7.7.43 | 0.6 | - |
CTP | wild-type, pH 7.5, 22°C | Neisseria meningitidis |