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Literature summary extracted from

  • Helmetag, V.; Samel, S.A.; Thomas, M.G.; Marahiel, M.A.; Essen, L.O.
    Structural basis for the erythro-stereospecificity of the L-arginine oxygenase VioC in viomycin biosynthesis (2009), FEBS J., 276, 3669-3682.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.41 expression in Escherichia coli BL21 Streptomyces vinaceus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.11.41 sitting drop vapor-diffusion method. The crystal structures of VioC are determined in complexes with the cofactor Fe(II), the substrate L-arginine, the product (2S,3S)-hydroxyarginine and the coproduct succinate at 1.1–1.3 A resolution Streptomyces vinaceus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.41 1.16
-
 L-canavanine pH 8.0, 30°C Streptomyces vinaceus
1.14.11.41 3.4
-
 L-arginine pH 8.0, 30°C Streptomyces vinaceus
1.14.11.41 7.05
-
 L-homoarginine pH 8.0, 30°C Streptomyces vinaceus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.41 Fe2+ Fe2+-dependent enzyme Streptomyces vinaceus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.41 41600
-
 x * 41600, SDS-PAGE Streptomyces vinaceus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.41 Streptomyces vinaceus Q6WZB0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.41 expressed as a fusion protein with an N-terminal hexahistidine tag in Escherichia coli BL21(DE3) Streptomyces vinaceus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.41 L-arginine + 2-oxoglutarate + O2 L-arginine is the preferred substrate Streptomyces vinaceus (3S)-3-hydroxy-L-arginine + succinate + CO2
-
 ?
1.14.11.41 L-canavanine + 2-oxoglutarate + O2
-
 Streptomyces vinaceus ? + succinate + CO2
-
 ?
1.14.11.41 L-homoarginine + 2-oxoglutarate + O2
-
 Streptomyces vinaceus (3S)-N6-(diaminomethylidene)-3-hydroxy-L-lysine + succinate + CO2
-
 ?
1.14.11.41 additional information no activity with D-arginine, NG-methyl-L-arginine, NG-hydroxynor-L-arginine Streptomyces vinaceus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.41 ? x * 41600, SDS-PAGE Streptomyces vinaceus

Synonyms

EC Number Synonyms Comment Organism
1.14.11.41 VioC
-
 Streptomyces vinaceus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.41 30
-
 assay at Streptomyces vinaceus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.11.41 1.22
-
 L-canavanine pH 8.0, 30°C Streptomyces vinaceus
1.14.11.41 13.85
-
 L-homoarginine pH 8.0, 30°C Streptomyces vinaceus
1.14.11.41 43.5
-
 L-arginine pH 8.0, 30°C Streptomyces vinaceus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.41 8
-
 assay at Streptomyces vinaceus

General Information

EC Number General Information Comment Organism
1.14.11.41 physiological function the enzyme is involved in the biosynthesis of the tuberactinomycin antibiotic viomycin Streptomyces vinaceus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.11.41 1.05
-
 L-canavanine pH 8.0, 30°C Streptomyces vinaceus
1.14.11.41 1.96
-
 L-homoarginine pH 8.0, 30°C Streptomyces vinaceus
1.14.11.41 12.8
-
 L-arginine pH 8.0, 30°C Streptomyces vinaceus