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Literature summary extracted from

  • Kyle, B.D.; Hurst, S.; Swayze, R.D.; Sheng, J.; Braun, A.P.
    Specific phosphorylation sites underlie the stimulation of a large conductance, Ca2+-activated K+ channel by cGMP-dependent protein kinase (2013), FASEB J., 27, 2027-2038.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.12 cGMP activates cGKI Rattus norvegicus
2.7.11.12 N2,2'-O-dibutyryl-cGMP a membrane-permeable cGMP analogue, activates cGKI Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.12 transient co-expression of cGKI and substrate BKCa channels in HEK 293 cells Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.12 Bos taurus
-
-
-
2.7.11.12 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.12 aorta
-
 Rattus norvegicus
-
2.7.11.12 smooth muscle cell aortic A7r5 smooth muscle cells Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.12 ATP + BKCa channel protein phosphorylation at mutiple serine residues, recombinant expressed Mus musculus BKCa channels Bos taurus ADP + BKCa channel phosphoprotein
-
 ?
2.7.11.12 ATP + BKCa channel protein phosphorylation at Ser691, Ser873, and Ser1112 on recombinant Mus musculus BKCa channels expressed in Rattus norvegicus aortic A7r5 smooth muscle cells or in humn in HEK 293 cells Rattus norvegicus ADP + BKCa channel phosphoprotein
-
 ?
2.7.11.12 additional information serine to alanine substitutions at 3 of 6 putative cGKI phosphorylation sites, Ser691, Ser873, and Ser1112, in the BKCa alpha-subunit and mutant expression in A7r5 cells individually reduce direct channel phosphorylation by 25-60% and block BKCa activation by either an NO donor or a membrane-permeable cGMP by 80-100% Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.12 cGKI
-
 Rattus norvegicus
2.7.11.12 cGKIalpha
-
 Bos taurus
2.7.11.12 type I cGMP-dependent protein kinase
-
 Rattus norvegicus
2.7.11.12 type Ialpha cGMP-dependent protein kinase
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.12 ATP
-
 Rattus norvegicus
2.7.11.12 ATP
-
 Bos taurus

General Information

EC Number General Information Comment Organism
2.7.11.12 malfunction acute inhibition of cGKI prevented stimulus-evoked enhancement of BKCa channel activity Bos taurus
2.7.11.12 malfunction acute inhibition of cGKI prevents stimulus-evoked enhancement of BKCa channel activity Rattus norvegicus
2.7.11.12 physiological function the activity of large conductance, Ca2+-activated K+ channels, i.e. BKCa channels, can be increased after modulation by type I cGMP-dependent protein kinase via nitric oxide/cGMP signaling Rattus norvegicus
2.7.11.12 physiological function the activity of large conductance, Ca2+-activated K+ channels, i.e. BKCa channels, can be increased after modulation by type I cGMP-dependent protein kinase via nitric oxide/cGMP signaling Bos taurus