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Literature summary extracted from

  • Shan, S.; Chen, X.; Liu, T.; Zhao, H.; Rao, Z.; Lou, Z.
    Crystal structure of 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (IspE) from Mycobacterium tuberculosis (2011), FASEB J., 25, 1577-1584.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.148
-
 Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.148 free enzyme, in complex with substrates 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol, ADP and with adenyl imidodiphosphate, to 1.0, 1.54, 1.0, and 1.0 A resolution, respectively. The structures present a characteristic galactose/homoserine/mevalonate/phosphomevalonate kinase superfamily alpha/beta-fold with a catalytic center located in a cleft between two domains and display clear substrate and ATP binding pockets Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.148 Mycobacterium tuberculosis P9WKG7
-
-
2.7.1.148 Mycobacterium tuberculosis H37Rv P9WKG7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.148 recombinant enzyme Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 Mycobacterium tuberculosis ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 Mycobacterium tuberculosis H37Rv ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?