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Literature summary extracted from

  • Zheng, R.; Matsui, E.; Shen, Y.; Musti, K.V.; Feng, Y.; Darnis, S.; Kawarabayasi, Y.; Kikuchi, H.; Harata, K.; Matsui, I.
    The novel function of a short region K253xRxxxD259 conserved in the exonuclease domain of hyperthermostable DNA polymerase I from Pyrococcus horikoshii (2001), Extremophiles, 5, 111-117.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.7 D259E moderate decrease of the polymerizing activity Pyrococcus horikoshii
2.7.7.7 D259G moderate decrease of the polymerizing activity Pyrococcus horikoshii
2.7.7.7 D259K the exonuclease activity of the mutant enzyme decreases drastically to 0.58% compared with that of the wild-type DNA polymerase Pyrococcus horikoshii
2.7.7.7 D259N moderate decrease of the polymerizing activity Pyrococcus horikoshii
2.7.7.7 K253E exonuclease activity of mutant increases 2.7fold compared to wild-type activity Pyrococcus horikoshii
2.7.7.7 K253E/R255E exonuclease activity of mutant increases 1.8fold compared to wild-type activity Pyrococcus horikoshii
2.7.7.7 R255D exonuclease activity of mutant increases 2.9fold compared to wild-type activity Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 Mg2+ the negative charge and the side-chain length of D259 might play a supporting role in coordinating the conserved Mg2+ to the correct position at the active center in the exonuclease domain Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Pyrococcus horikoshii O59610
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Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
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 Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn the functional motif, K253xRxxxD259 (outside known motifs Exo I, II, and III), that is important not only for exonuclease activity but also for polymerizing activity, confirms functional interdependence between the polymerase and exonuclease domains. The short loop region, K253G254R255, probably contributes to binding to DNA substrates Pyrococcus horikoshii diphosphate + DNAn+1
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DNA polymerase I
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 Pyrococcus horikoshii