BRENDA - Enzyme Database

Thermal stability and biochemical properties of isocitrate dehydrogenase from the thermoacidophilic archaeon Thermoplasma acidophilum

Stokke, R.; Birkeland, N.K.; Steen, I.H.; Extremophiles 11, 397-402 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
expressed in Escherichia coli BL21
Thermoplasma acidophilum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.111
-
NADP+
60°C, pH 7.5
Thermoplasma acidophilum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Thermoplasma acidophilum
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Thermoplasma acidophilum
Q9HLV8
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Thermoplasma acidophilum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
722204
Thermoplasma acidophilum
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
70
-
-
Thermoplasma acidophilum
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.1.42
50
80
50°C: about 50% of maximal activity, 80°C: about 65% of maximal activity
Thermoplasma acidophilum
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
60
-
pH 5, enzyme retains 91% of its activity
Thermoplasma acidophilum
1.1.1.42
70
-
half-life: 88 min
Thermoplasma acidophilum
1.1.1.42
75
-
half-life: 24 min
Thermoplasma acidophilum
1.1.1.42
82
-
melting temperature at pH 7.5 is 82.2°C
Thermoplasma acidophilum
1.1.1.42
85
-
melting temperature at pH 5.8 is 84.5°C
Thermoplasma acidophilum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.5
-
-
Thermoplasma acidophilum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
5.8
7.5
three-fold reduction in activity when the pH is shifted from the pH-optimum at 7.5 to pH 5.8
Thermoplasma acidophilum
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.42
5
-
1 h, 60°C, enzyme retains 91% of its activity
Thermoplasma acidophilum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
no activity when NAD+ up to 2 mM
Thermoplasma acidophilum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
expressed in Escherichia coli BL21
Thermoplasma acidophilum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
no activity when NAD+ up to 2 mM
Thermoplasma acidophilum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.111
-
NADP+
60°C, pH 7.5
Thermoplasma acidophilum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Thermoplasma acidophilum
-
2-oxoglutarate + CO2 + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Thermoplasma acidophilum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
722204
Thermoplasma acidophilum
2-oxoglutarate + CO2 + NADPH + H+
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
70
-
-
Thermoplasma acidophilum
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.1.1.42
50
80
50°C: about 50% of maximal activity, 80°C: about 65% of maximal activity
Thermoplasma acidophilum
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
60
-
pH 5, enzyme retains 91% of its activity
Thermoplasma acidophilum
1.1.1.42
70
-
half-life: 88 min
Thermoplasma acidophilum
1.1.1.42
75
-
half-life: 24 min
Thermoplasma acidophilum
1.1.1.42
82
-
melting temperature at pH 7.5 is 82.2°C
Thermoplasma acidophilum
1.1.1.42
85
-
melting temperature at pH 5.8 is 84.5°C
Thermoplasma acidophilum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.5
-
-
Thermoplasma acidophilum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
5.8
7.5
three-fold reduction in activity when the pH is shifted from the pH-optimum at 7.5 to pH 5.8
Thermoplasma acidophilum
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.42
5
-
1 h, 60°C, enzyme retains 91% of its activity
Thermoplasma acidophilum