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Literature summary extracted from

  • Stokke, R.; Birkeland, N.K.; Steen, I.H.
    Thermal stability and biochemical properties of isocitrate dehydrogenase from the thermoacidophilic archaeon Thermoplasma acidophilum (2007), Extremophiles, 11, 397-402.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 expressed in Escherichia coli BL21 Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.111
-
NADP+ 60°C, pH 7.5 Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Thermoplasma acidophilum
-
2-oxoglutarate + CO2 + NADPH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Thermoplasma acidophilum Q9HLV8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42
-
Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
Thermoplasma acidophilum 2-oxoglutarate + CO2 + NADPH + H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 TaIDH
-
Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.42 70
-
-
Thermoplasma acidophilum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.42 50 80 50°C: about 50% of maximal activity, 80°C: about 65% of maximal activity Thermoplasma acidophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.42 60
-
pH 5, enzyme retains 91% of its activity Thermoplasma acidophilum
1.1.1.42 70
-
half-life: 88 min Thermoplasma acidophilum
1.1.1.42 75
-
half-life: 24 min Thermoplasma acidophilum
1.1.1.42 82
-
melting temperature at pH 7.5 is 82.2°C Thermoplasma acidophilum
1.1.1.42 85
-
melting temperature at pH 5.8 is 84.5°C Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 7.5
-
-
Thermoplasma acidophilum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.42 5.8 7.5 three-fold reduction in activity when the pH is shifted from the pH-optimum at 7.5 to pH 5.8 Thermoplasma acidophilum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.42 5
-
1 h, 60°C, enzyme retains 91% of its activity Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+ no activity when NAD+ up to 2 mM Thermoplasma acidophilum