EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.42 | expressed in Escherichia coli BL21 | Thermoplasma acidophilum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.42 | 0.111 | - |
NADP+ | 60°C, pH 7.5 | Thermoplasma acidophilum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.42 | isocitrate + NADP+ | Thermoplasma acidophilum | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.42 | Thermoplasma acidophilum | Q9HLV8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.42 | - |
Thermoplasma acidophilum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.42 | isocitrate + NADP+ | - |
Thermoplasma acidophilum | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.42 | TaIDH | - |
Thermoplasma acidophilum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 70 | - |
- |
Thermoplasma acidophilum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 50 | 80 | 50°C: about 50% of maximal activity, 80°C: about 65% of maximal activity | Thermoplasma acidophilum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 60 | - |
pH 5, enzyme retains 91% of its activity | Thermoplasma acidophilum |
1.1.1.42 | 70 | - |
half-life: 88 min | Thermoplasma acidophilum |
1.1.1.42 | 75 | - |
half-life: 24 min | Thermoplasma acidophilum |
1.1.1.42 | 82 | - |
melting temperature at pH 7.5 is 82.2°C | Thermoplasma acidophilum |
1.1.1.42 | 85 | - |
melting temperature at pH 5.8 is 84.5°C | Thermoplasma acidophilum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 7.5 | - |
- |
Thermoplasma acidophilum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 5.8 | 7.5 | three-fold reduction in activity when the pH is shifted from the pH-optimum at 7.5 to pH 5.8 | Thermoplasma acidophilum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 5 | - |
1 h, 60°C, enzyme retains 91% of its activity | Thermoplasma acidophilum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.42 | NADP+ | no activity when NAD+ up to 2 mM | Thermoplasma acidophilum |