EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.35 | expressed in Escherichia coli Rosetta (DE3) cells | Bombyx mori |
EC Number | General Stability | Organism |
---|---|---|
2.7.1.35 | if instead of the phosphate buffer an acetate buffer is used, enzymatic activity is reduced to 74% and almost no activity is recorded when a citrate buffer is used | Bombyx mori |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.35 | 0.0441 | - |
pyridoxal | in 70 mM potassium phosphate (pH 5.5), 0.5 mM ZnCl2, at 37°C | Bombyx mori | |
2.7.1.35 | 0.0579 | - |
ATP | in 70 mM potassium phosphate (pH 5.5), 0.5 mM ZnCl2, at 37°C | Bombyx mori |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.35 | Ca2+ | - |
Bombyx mori | |
2.7.1.35 | Co2+ | - |
Bombyx mori | |
2.7.1.35 | Fe2+ | - |
Bombyx mori | |
2.7.1.35 | K+ | when only triethanolamine is present as the cation, K+ is an activator of the enzyme | Bombyx mori | |
2.7.1.35 | Mn2+ | - |
Bombyx mori | |
2.7.1.35 | Zn2+ | Zn2+ is the most effective cation for catalysis under saturating substrate concentrations | Bombyx mori |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.35 | 33900 | - |
2 * 33900, SDS-PAGE | Bombyx mori |
2.7.1.35 | 68000 | - |
gel filtration | Bombyx mori |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.35 | Bombyx mori | Q1PCB1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.35 | Ni Sepharose affinity column chromatography | Bombyx mori |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.35 | 0.07 | - |
crude enzyme, in 70 mM potassium phosphate (pH 5.5), 0.5 mM ZnCl2, at 37°C | Bombyx mori |
2.7.1.35 | 1.8 | - |
crude enzyme, in 70 mM potassium phosphate (pH 5.5), 0.5 mM ZnCl2, at 37°C | Bombyx mori |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.35 | ATP + pyridoxal | - |
Bombyx mori | ADP + pyridoxal 5'-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.35 | homodimer | 2 * 33900, SDS-PAGE | Bombyx mori |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.35 | PLK | - |
Bombyx mori |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.35 | 50 | - |
- |
Bombyx mori |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.35 | 40 | - |
greatest stability is at below 40°C | Bombyx mori |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.35 | 1.23 | - |
ATP | in 70 mM potassium phosphate (pH 5.5), 0.5 mM ZnCl2, at 37°C | Bombyx mori | |
2.7.1.35 | 1.35 | - |
pyridoxal | in 70 mM potassium phosphate (pH 5.5), 0.5 mM ZnCl2, at 37°C | Bombyx mori |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.35 | 5.5 | 6 | - |
Bombyx mori |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.35 | 4.5 | 8.5 | the enzyme is inactive below pH 4.5. Enzyme activity decreases slowly above pH 6.0, to approximately 35% at pH 8.5 | Bombyx mori |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.1.35 | Bombyx mori | calculated from amino acid sequence | - |
6.3 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.1.35 | physiological function | pyridoxal kinase is a key enzyme in the metabolism of vitamin B6 | Bombyx mori |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.35 | 21.2 | - |
ATP | in 70 mM potassium phosphate (pH 5.5), 0.5 mM ZnCl2, at 37°C | Bombyx mori | |
2.7.1.35 | 30.6 | - |
pyridoxal | in 70 mM potassium phosphate (pH 5.5), 0.5 mM ZnCl2, at 37°C | Bombyx mori |