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Literature summary extracted from

  • Tokunaga, H.; Izutsu, K.; Arai, S.; Yonezawa, Y.; Kuroki, R.; Arakawa, T.; Tokunaga, M.
    Dimer-tetramer assembly of nucleoside diphosphate kinase from moderately halophilic bacterium Chromohalobacter salexigens DSM3043: Both residues 134 and 136 are critical for the tetramer assembly (2010), Enzyme Microb. Technol., 46, 129-135.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.6 expressed in Escherichia coli BL21star(DE3) cells Chromohalobacter salexigens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.6 C139S the mutant forms a dimer Chromohalobacter salexigens
2.7.4.6 E136T the mutant forms a dimer Chromohalobacter salexigens
2.7.4.6 G134A/E136T the mutant forms a tetramer Chromohalobacter salexigens
2.7.4.6 G134A/E136T/C139S the mutant forms a tetramer Chromohalobacter salexigens
2.7.4.6 G134A/N135A/E136T the mutant forms a tetramer Chromohalobacter salexigens
2.7.4.6 G134E the mutant forms a dimer Chromohalobacter salexigens
2.7.4.6 G134E/E136T the mutant forms a dimer Chromohalobacter salexigens
2.7.4.6 G134EA the mutant forms a dimer Chromohalobacter salexigens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.6 15159
-
 2 * 15159, calculated from amino acid sequence Chromohalobacter salexigens
2.7.4.6 33000
-
 gel filtration Chromohalobacter salexigens

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Chromohalobacter salexigens Q1QTL2
-
-
2.7.4.6 Chromohalobacter salexigens DSM 3043 Q1QTL2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 ATP-agarose-affinity column chromatography, and gel filtration Chromohalobacter salexigens

Subunits

EC Number Subunits Comment Organism
2.7.4.6 homodimer 2 * 15159, calculated from amino acid sequence Chromohalobacter salexigens

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 NDK
-
 Chromohalobacter salexigens
2.7.4.6 nucleoside diphosphate kinase
-
 Chromohalobacter salexigens