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Literature summary extracted from
- Expression and extensive characterization of a beta-glycosidase from the extreme thermoacidophilic archaeon Sulfolobus solfataricus in Escherichia coli: authenticity of the recombinant enzyme (1995), Enzyme Microb. Technol., 17, 992-997.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.B26 | expression in Escherichia coli | Saccharolobus solfataricus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.B26 | 0.4 | - |
4-nitrophenyl beta-D-fucopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus |  |
| 3.2.1.B26 | 0.45 | - |
4-nitrophenyl beta-D-glucopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 0.45 | - |
4-nitrophenyl beta-D-fucopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 0.6 | - |
4-nitrophenyl beta-D-glucopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 1.29 | - |
laminaribiose | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 1.4 | - |
laminaribiose | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 1.5 | - |
2-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 1.99 | - |
2-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 2.33 | - |
4-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 2.51 | - |
4-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 21.54 | - |
cellobiose | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 22.4 | - |
cellobiose | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.B26 | Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.B26 | - |
Saccharolobus solfataricus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.B26 | -20°C, in 50 mM sodium phosphate buffer, pH 7.0, 50% glycerol, no appreciable loss of activity even after several months | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.B26 | 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
| 3.2.1.B26 | 4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
| 3.2.1.B26 | 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
| 3.2.1.B26 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.B26 | cellobiose + H2O | - |
Saccharolobus solfataricus | 2 beta-D-glucose | - |
? | |
| 3.2.1.B26 | cellotetraose + H2O | - |
Saccharolobus solfataricus | cellotriose + D-glucose | - |
? | |
| 3.2.1.B26 | laminaribiose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
| 3.2.1.B26 | additional information | broad specificity and remarkable glucan-1,4-beta-D-glucosidase (exocellobiase) activity | Saccharolobus solfataricus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.B26 | Sbeta-gly | - |
Saccharolobus solfataricus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.B26 | 70 | 80 | activity at 70°C is about 45% compared to the activity at 85°C. The temperatureactivity profiles of native and recombinant enzyme are almost indistinguishable using 2-nitrophenyl-beta-D-galactopyranoside | Saccharolobus solfataricus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.B26 | 30 | 100 | pH 6.5, no denaturation occurrs in the temperature range of 30 to 100°C for both the native and recombinant enzyme | Saccharolobus solfataricus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.B26 | 427 | - |
4-nitrophenyl beta-D-fucopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 460 | - |
4-nitrophenyl beta-D-fucopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 524 | - |
laminaribiose | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 547 | - |
cellobiose | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 573 | - |
laminaribiose | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 634 | - |
4-nitrophenyl beta-D-glucopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 654 | - |
cellobiose | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 726 | - |
4-nitrophenyl beta-D-glucopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 930 | - |
4-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 1252 | - |
2-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 1341 | - |
4-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 1416 | - |
2-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.B26 | 6.5 | - |
no denaturation occurrs in the temperature range of 30 to 100°C for both the native and recombinant enzyme | Saccharolobus solfataricus |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.B26 | 25 | - |
cellobiose | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 29 | - |
cellobiose | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 370 | - |
4-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 407 | - |
laminaribiose | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 410 | - |
laminaribiose | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 576 | - |
4-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 712 | - |
2-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 844 | - |
2-nitrophenyl beta-D-galactopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 941 | - |
4-nitrophenyl beta-D-fucopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 1058 | - |
4-nitrophenyl beta-D-glucopyranoside | pH and temperature not specified in the publication, recombinant enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 1151 | - |
4-nitrophenyl beta-D-fucopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
| 3.2.1.B26 | 1633 | - |
4-nitrophenyl beta-D-glucopyranoside | pH and temperature not specified in the publication, native enzyme | Saccharolobus solfataricus | |
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