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Literature summary extracted from

  • Furukawa, A.; Nagata, T.; Matsugami, A.; Habu, Y.; Sugiyama, R.; Hayashi, F.; Kobayashi, N.; Yokoyama, S.; Takaku, H.; Katahira, M.
    Structure, interaction and real-time monitoring of the enzymatic reaction of wild-type APOBEC3G (2009), EMBO J., 28, 440-451.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.B9 expressed in Escherichia coli BL21(DE3/RIL) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.B9 F202A the mutation causes a decrease of the enzyme activity Homo sapiens
3.5.4.B9 F241K the mutation causes a decrease of the enzyme activity Homo sapiens
3.5.4.B9 L235A the mutation causes a decrease of the enzyme activity Homo sapiens
3.5.4.B9 L235K the mutation causes a decrease of the enzyme activity Homo sapiens
3.5.4.B9 L242A the mutation causes a decrease of the enzyme activity Homo sapiens
3.5.4.B9 L242K the mutation causes a decrease of the enzyme activity Homo sapiens
3.5.4.B9 T203A the mutation causes a decrease of the enzyme activity Homo sapiens
3.5.4.B9 V233A the mutation causes a decrease of the enzyme activity Homo sapiens
3.5.4.B9 V233K the mutation causes a decrease of the enzyme activity Homo sapiens
3.5.4.B9 W232A the mutation causes a decrease of the enzyme activity Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.B9 Homo sapiens Q9HC16
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.B9 Ni Sepharose resin column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.B9 cytosine in single-stranded viral DNA + H2O minus strand deamination occurs preferentially at a CCCA sequence. The third cytidine of the d(CCCA) segment is deaminated at an early stage and that then the second one is deaminated at a late stage, so the deamination is carried out in a strict 3'-5' order Homo sapiens uracil in single-stranded viral DNA + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.B9 APOBEC3G
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
3.5.4.B9 physiological function the enzyme exhibits anti-human immunodeficiency virus-1 (HIV-1) activity by deaminating cytidines of the minus strand of HIV-1. Virus infectivity factor of HIV-1 counteracts the anti-HIV-1 activity of the enzyme Homo sapiens