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Literature summary extracted from

  • Peneff, C.; Ferrari, P.; Charrier, V.; Taburet, Y.; Monnier, C.; Zamboni, V.; Winter, J.; Harnois, M.; Fassy, F.; Bourne, Y.
    Crystal structures of two human pyrophosphorylase isoforms in complexes with UDPGlc(Gal)NAc: Role of the alternatively spliced insert in the enzyme oligomeric assembly and active site architecture (2001), EMBO J., 20, 6191-6202.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.83 expressed in Escherichia coli XL1 Blue Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.83 vapor diffusion technique, PEG600, 20°C, pH5.7 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.83 R115A activity drastically affected Homo sapiens
2.7.7.83 R115A activity drastically affected, no dimer formation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.83 0.0053
-
 N-acetylglucosamine 1-phosphate wild type protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 0.006
-
 N-acetylglucosamine 1-phosphate wild type protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 0.032
-
 UDP-N-acetylglucosamine wild type protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 0.049
-
 UTP wild type protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 0.053
-
 UTP wild type protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 0.5
-
 UDP-N-acetyl-alpha-D-galactosamine wild type protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 0.53
-
 UDP-N-acetyl-alpha-D-galactosamine wild type protein, pH 8.0, 30°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.83 74000
-
 gel filtration Homo sapiens
2.7.7.83 74000
-
 2 * 74000, gel filtration Homo sapiens
2.7.7.83 120000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.83 Homo sapiens
-
-
-
2.7.7.83 Homo sapiens Q16222
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.83 anion exchange, phenyl interaction and gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.83 astrocyte
-
 Homo sapiens
-
2.7.7.83 cartilage
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.83 0.9
-
 R115A mutant protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 1.5
-
 R115A mutant protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 68
-
 wild type protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 69
-
 wild type protein, pH 8.0, 30°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.83 diphosphate + UDP-N-acetyl-alpha-D-galactosamine
-
 Homo sapiens UTP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 r
2.7.7.83 diphosphate + UDP-N-acetylglucosamine
-
 Homo sapiens UTP + N-acetylglucosamine 1-phosphate
-
 r
2.7.7.83 UTP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 Homo sapiens diphosphate + UDP-N-acetyl-alpha-D-galactosamine
-
 r
2.7.7.83 UTP + N-acetylglucosamine 1-phosphate
-
 Homo sapiens diphosphate + UDP-N-acetylglucosamine
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.83 homodimer 2 * 74000, gel filtration Homo sapiens
2.7.7.83 monomer 74000, gel filtration Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.7.83 N-acetylglucosamine 1-phosphate uridyltransferase
-
 Homo sapiens
2.7.7.83 UAP1
-
 Homo sapiens
2.7.7.83 UDP-N-acetylglucosamine pyrophosphorylase AGX1 alternatively spliced isoform to AGX2, differs from those referenced in the literature by one change: S445G (most probably a gene polymorphism) Homo sapiens
2.7.7.83 UDP-N-acetylglucosamine pyrophosphorylase AGX2 alternatively spliced isoform to AGX1, differs from those referenced in the literature by two changes: S445G and Q454S (most probably a gene polymorphism) Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.83 180
-
 UDP-N-acetyl-alpha-D-galactosamine wild type protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 200
-
 UDP-N-acetyl-alpha-D-galactosamine wild type protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 1200
-
 UTP wild type protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 1400
-
 UTP wild type protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 2000
-
 UDP-N-acetylglucosamine wild type protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 2200
-
 UDP-N-acetylglucosamine wild type protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 11200
-
 N-acetylglucosamine 1-phosphate wild type protein, pH 8.0, 30°C Homo sapiens
2.7.7.83 12200
-
 N-acetylglucosamine 1-phosphate wild type protein, pH 8.0, 30°C Homo sapiens