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Literature summary extracted from

  • Dieni, C.A.; Storey, K.B.
    Regulation of hexokinase by reversible phosphorylation in skeletal muscle of a freeze-tolerant frog (2011), Comp. Biochem. Physiol. B, 159, 236-243.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 2-Phosphoglycerol
-
Lithobates sylvaticus
2.7.1.1 Urea
-
Lithobates sylvaticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.139
-
D-glucose control, pH 8.2, 5°C, no glucose Lithobates sylvaticus
2.7.1.1 0.144
-
D-glucose control, pH 8.2, 25°C Lithobates sylvaticus
2.7.1.1 0.144
-
D-glucose control, pH 8.2, 25°C, no glucose Lithobates sylvaticus
2.7.1.1 0.239
-
ATP control, pH 8.2, 25°C, 250 mM glucose Lithobates sylvaticus
2.7.1.1 0.248
-
ATP control, pH 8.2, 25°C Lithobates sylvaticus
2.7.1.1 0.248
-
ATP control, pH 8.2, 25°C, no glucose Lithobates sylvaticus
2.7.1.1 0.248
-
D-glucose frozen, pH 8.2, 25°C Lithobates sylvaticus
2.7.1.1 0.248
-
D-glucose frozen, pH 8.2, 25°C, no glucose Lithobates sylvaticus
2.7.1.1 0.253
-
D-glucose frozen, pH 8.2, 5°C, no glucose Lithobates sylvaticus
2.7.1.1 0.257
-
ATP control, pH 8.2, 5°C, 250 mM glucose Lithobates sylvaticus
2.7.1.1 0.33
-
ATP frozen, pH 8.2, 25°C Lithobates sylvaticus
2.7.1.1 0.33
-
ATP frozen, pH 8.2, 25°C, no glucose Lithobates sylvaticus
2.7.1.1 0.336
-
ATP frozen, pH 8.2, 5°C, 250 mM glucose Lithobates sylvaticus
2.7.1.1 0.342
-
ATP frozen, pH 8.2, 25°C, 250 mM glucose Lithobates sylvaticus
2.7.1.1 0.364
-
ATP control, pH 8.2, 5°C, no glucose Lithobates sylvaticus
2.7.1.1 0.505
-
ATP frozen, pH 8.2, 5°C, no glucose Lithobates sylvaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.1 100000
-
SDS-PAGE Lithobates sylvaticus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Lithobates sylvaticus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.1 phosphoprotein differential phosphorylation of hexokinase is responsible for altered kinetic properties: in control frogs, hexokinase is in a higher phosphate form and displays a high substrate affinity and high activity, whereas in frozen frogs hexokinase is less phosphorylated, with lower substrate affinity and lower activity Lithobates sylvaticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.1 muscle
-
Lithobates sylvaticus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + D-glucose
-
Lithobates sylvaticus ADP + D-glucose 6-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.1 hexokinase
-
Lithobates sylvaticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.1 25
-
assay at Lithobates sylvaticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 8
-
optimal pH Lithobates sylvaticus
2.7.1.1 8.2
-
assay at Lithobates sylvaticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.1 7.9 8.4 90% activity, frozen frogs Lithobates sylvaticus
2.7.1.1 8.1 8.6 90% activity, control frogs Lithobates sylvaticus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.1 0.0045
-
frozen, pH 8.2, 25°C Lithobates sylvaticus Urea
2.7.1.1 0.0053
-
control, pH 8.2, 25°C Lithobates sylvaticus Urea