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Literature summary extracted from

  • Smutok, O.; Broda, D.; Smutok, H.; Dmytruk, K.; Gonchar, M.
    Chromate-reducing activity of Hansenula polymorpha recombinant cells over-producing flavocytochrome b2 (2011), Chemosphere, 83, 449-454.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.2.3 environmental protection the reductive pathway of the enzyme resulting in formation of less toxic Cr(III)-species is suggested to be the most important among possible mechanisms for chromate biodetoxification Ogataea angusta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.3 2.09
-
 (S)-lactate pH 7.0, 20°C Ogataea angusta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c Ogataea angusta
-
 pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 (S)-lactate + 2 ferricytochrome c Ogataea angusta C-105
-
 pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 additional information Ogataea angusta chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol ?
-
 ?
1.1.2.3 additional information Ogataea angusta C-105 chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Ogataea angusta
-
-
-
1.1.2.3 Ogataea angusta C-105
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.3 native enzyme to homogeneity Ogataea angusta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.3 9
-
 purified native enzyme, pH 7.5, 20°C Ogataea angusta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c
-
 Ogataea angusta pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 (S)-lactate + 2 ferricytochrome c
-
 Ogataea angusta C-105 pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 (S)-lactate + K3[Fe(CN)6]
-
 Ogataea angusta ?
-
 ?
1.1.2.3 (S)-lactate + K3[Fe(CN)6]
-
 Ogataea angusta C-105 ?
-
 ?
1.1.2.3 additional information chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol Ogataea angusta ?
-
 ?
1.1.2.3 additional information the enzyme has absolute specificity for L-lactate, yet is non-selective with respect to its electron acceptor Ogataea angusta ?
-
 ?
1.1.2.3 additional information chromate-reducing ability of enzyme-overproducing recombinant cells, the highest chromate-reducing activity of the cells is achieved in the presence of 2,6-dichlorophenolindophenol Ogataea angusta C-105 ?
-
 ?
1.1.2.3 additional information the enzyme has absolute specificity for L-lactate, yet is non-selective with respect to its electron acceptor Ogataea angusta C-105 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.2.3 FC b2
-
 Ogataea angusta
1.1.2.3 flavocytochrome b2
-
 Ogataea angusta
1.1.2.3 L-lactate:cytochrome c-oxidoreductase
-
 Ogataea angusta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.3 20 24 assay at Ogataea angusta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.3 6.3 7.5 assay at Ogataea angusta

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.3 2,6-dichlorophenolindophenol
-
 Ogataea angusta
1.1.2.3 ferricytochrome c
-
 Ogataea angusta