BRENDA - Enzyme Database show

Redox chemistry of biological tungsten: an EPR study of the aldehyde oxidoreductase from Pyrococcus furiosus

Arendsen, A.F.; de Vocht, M.; Bulsink, Y.B.M.; Hagen, W.R.; Chemistry 1, 292-296 (1996)
No PubMed abstract available

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.7.5
Iron
each subunit carries one [4Fe-4S] cubane and a novel tungsten cofactor containing two pterins. A single iron atom bridges between the subunits. The active-site redox chemistry is based on the pterin part of the cofactor
Pyrococcus furiosus
1.2.7.5
Tungsten
each subunit carries one [4Fe-4S] cubane and a novel tungsten cofactor containing two pterins. A single iron atom bridges between the subunits
Pyrococcus furiosus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.5
80000
-
2 * 80000, SDS-PAGE
Pyrococcus furiosus
1.2.7.5
110000
-
gel filtration
Pyrococcus furiosus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.7.5
Pyrococcus furiosus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.7.5
-
Pyrococcus furiosus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.7.5
10
-
65°C, pH not specified in the publication
Pyrococcus furiosus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.5
crotonaldehyde + H2O + oxidized ferredoxin
-
722057
Pyrococcus furiosus
crotonate + reduced ferredoxin
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.7.5
homodimer
2 * 80000, SDS-PAGE
Pyrococcus furiosus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.7.5
Iron
each subunit carries one [4Fe-4S] cubane and a novel tungsten cofactor containing two pterins. A single iron atom bridges between the subunits. The active-site redox chemistry is based on the pterin part of the cofactor
Pyrococcus furiosus
1.2.7.5
Tungsten
each subunit carries one [4Fe-4S] cubane and a novel tungsten cofactor containing two pterins. A single iron atom bridges between the subunits
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.7.5
80000
-
2 * 80000, SDS-PAGE
Pyrococcus furiosus
1.2.7.5
110000
-
gel filtration
Pyrococcus furiosus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.7.5
-
Pyrococcus furiosus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.7.5
10
-
65°C, pH not specified in the publication
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.7.5
crotonaldehyde + H2O + oxidized ferredoxin
-
722057
Pyrococcus furiosus
crotonate + reduced ferredoxin
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.7.5
homodimer
2 * 80000, SDS-PAGE
Pyrococcus furiosus