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Literature summary extracted from

  • Hamed, R.B.; Mecinovic, J.; Ducho, C.; Claridge, T.D.; Schofield. C.J.
    Carboxymethylproline synthase catalysed syntheses of functionalised N-heterocycles (2010), Chem. Commun. (Camb. ), 46, 1413-1415.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.226 medicine carboxymethylproline synthases is a biocatalysts for preparing functionalised N-heterocycles in a diastereoselective fashion. The products can be converted into the respective bicyclic beta-lactams of potential application in the semisynthesis of stable beta-lactam antibiotics Pectobacterium carotovorum
2.3.1.226 synthesis carboxymethylproline synthases is a biocatalysts for preparing functionalised N-heterocycles in a diastereoselective fashion. The products can be converted into the respective bicyclic beta-lactams of potential application in the semisynthesis of stable beta-lactam antibiotics Pectobacterium carotovorum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.226 H229A the CarB variant is able to form (2S,7S)-7-(carboxymethyl)-azepane-2-carboxylic acid from malonyl-CoA and L-aminopimelate semialdehyde in a yield of about 3 times higher than that obtained with wild-type CarB Pectobacterium carotovorum

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.226 Pectobacterium carotovorum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.226 malonyl-CoA + (S)-1-pyrroline-5-carboxylate + H2O
-
 Pectobacterium carotovorum CoA + (2S,5S)-5-carboxymethylproline + CO2
-
 ?
2.3.1.226 malonyl-CoA + L-2-aminopimelate semialdehyde the CarB His229Ala variant is able to form the target compound in a yield of about 3 times higher than that obtained with wild-type CarB Pectobacterium carotovorum CoA + (2S,7S)-7-(carboxymethyl)-azepane-2-carboxylic acid + CO2
-
 ?
2.3.1.226 additional information no activity with L-2-aminosuberate semialdehyde (wild-type enzyme and mutant enzyme H229A) Pectobacterium carotovorum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.226 CarB
-
 Pectobacterium carotovorum