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Literature summary extracted from

  • Xu, H.; Heide, L.; Li, S.M.
    New aminocoumarin antibiotics formed by a combined mutational and chemoenzymatic approach utilizing the carbamoyltransferase NovN (2004), Chem. Biol., 11, 655-662.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.12 overexpression in Streptomyces lividans as an N-terminal His6 fusion protein Streptomyces niveus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.12 0.0019
-
novclobiocin 104 pH 8.0, 30°C Streptomyces niveus
2.1.3.12 0.0024
-
decarbamoylnovobiocin pH 8.0, 30°C Streptomyces niveus
2.1.3.12 0.0143
-
novclobiocin 105 pH 8.0, 30°C Streptomyces niveus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.3.12 Mg2+ the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM Streptomyces niveus
2.1.3.12 Mn2+ the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM Streptomyces niveus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.12 78500
-
1 * 78500, calculated from sequence Streptomyces niveus
2.1.3.12 79000
-
gel filtration Streptomyces niveus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.12 carbamoyl phosphate + decarbamoylnovobiocin Streptomyces niveus the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin phosphate + novobiocin
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.12 Streptomyces niveus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.12 N-terminal His6 fusion protein Streptomyces niveus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.12 carbamoyl phosphate + decarbamoylnovobiocin the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin Streptomyces niveus phosphate + novobiocin
-
?
2.1.3.12 carbamoyl phosphate + decarbamoylnovobiocin NovN is specific for carbamoyl phosphate as substrate. With acetyl phosphate, no product formation is observed Streptomyces niveus phosphate + novobiocin
-
?
2.1.3.12 carbamoyl phosphate + novclobiocin 104 87% of novclobiocin 104 is converted to the carbamoylated derivative after overnight incubation Streptomyces niveus phosphate + ?
-
?
2.1.3.12 carbamoyl phosphate + novclobiocin 105
-
Streptomyces niveus phosphate + novclobiocin 115
-
?
2.1.3.12 carbamoyl phosphate + novclobiocin 107
-
Streptomyces niveus phosphate + novclobiocin 117
-
?
2.1.3.12 carbamoyl phosphate + novclobiocin 108
-
Streptomyces niveus phosphate + novclobiocin 118
-
?
2.1.3.12 carbamoyl phosphate + novclobiocin 283
-
Streptomyces niveus phosphate + novclobiocin 284
-
?

Subunits

EC Number Subunits Comment Organism
2.1.3.12 monomer 1 * 78500, calculated from sequence Streptomyces niveus

Synonyms

EC Number Synonyms Comment Organism
2.1.3.12 NovN
-
Streptomyces niveus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.3.12 30
-
assay at Streptomyces niveus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.3.12 1.32
-
novclobiocin 104 pH 8.0, 30°C Streptomyces niveus
2.1.3.12 1.53
-
novclobiocin 105 pH 8.0, 30°C Streptomyces niveus
2.1.3.12 2.4
-
decarbamoylnovobiocin pH 8.0, 30°C Streptomyces niveus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.12 8
-
assay at Streptomyces niveus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.3.12 ATP the enzyme is strictly dependent on the presence ATP and divalent cations such as Mg2+ or Mn2+. Optimal concentrations of both Mg2+ and ATP is 2 mM Streptomyces niveus

General Information

EC Number General Information Comment Organism
2.1.3.12 physiological function the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin Streptomyces niveus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.3.12 107
-
novclobiocin 105 pH 8.0, 30°C Streptomyces niveus
2.1.3.12 695
-
novclobiocin 104 pH 8.0, 30°C Streptomyces niveus
2.1.3.12 1000
-
decarbamoylnovobiocin pH 8.0, 30°C Streptomyces niveus