EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.2 | expressed in Escherichia coli | Corynebacterium ammoniagenes |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.7.2 | mutant enzyme E268D, hanging drop vapor diffusion method | Corynebacterium ammoniagenes |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.26 | E268A | inactive | Corynebacterium ammoniagenes |
2.7.1.26 | E268D | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Corynebacterium ammoniagenes |
2.7.1.26 | N210A | inactive | Corynebacterium ammoniagenes |
2.7.1.26 | N210D | the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme | Corynebacterium ammoniagenes |
2.7.1.26 | T208A | inactive | Corynebacterium ammoniagenes |
2.7.1.26 | T208D | inactive | Corynebacterium ammoniagenes |
2.7.7.2 | E268A | the mutant shows increased catalytic efficiency for FMN and reduced catalytic efficiency for ATP compared to the wild type enzyme | Corynebacterium ammoniagenes |
2.7.7.2 | E268D | the mutant shows about wild type catalytic efficiencies for ATP and FMN | Corynebacterium ammoniagenes |
2.7.7.2 | N210A | the mutant shows strongly reduced catalytic efficiencies for FMN and ATP compared to the wild type enzyme | Corynebacterium ammoniagenes |
2.7.7.2 | N210D | the mutant shows strongly reduced catalytic efficiencies for FMN and ATP compared to the wild type enzyme | Corynebacterium ammoniagenes |
2.7.7.2 | T208A | the mutant shows increased catalytic efficiency for FMN and reduced catalytic efficiency for ATP compared to the wild type enzyme | Corynebacterium ammoniagenes |
2.7.7.2 | T208D | the mutant shows increased catalytic efficiency for FMN and increased catalytic efficiency for ATP compared to the wild type enzyme | Corynebacterium ammoniagenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.26 | 0.0021 | - |
riboflavin | apparent value, mutant enzyme N210D, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 0.0041 | - |
riboflavin | apparent value, mutant enzyme E268D, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 0.013 | - |
riboflavin | apparent value, wild type enzyme, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 0.0137 | - |
ATP | apparent value, wild type enzyme, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 0.0176 | - |
ATP | apparent value, mutant enzyme E268D, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 0.0453 | - |
ATP | apparent value, mutant enzyme N210D, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 0.00088 | - |
FMN | mutant enzyme E268A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 0.00088 | - |
FMN | mutant enzyme T208D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 0.00095 | - |
FMN | mutant enzyme T208A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 0.00117 | - |
FMN | mutant enzyme E268D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 0.00119 | - |
FMN | wild type enzyme, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 0.00823 | - |
FMN | mutant enzyme N210A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 0.01501 | - |
FMN | mutant enzyme N210D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 0.03568 | - |
ATP | wild type enzyme, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 0.0382 | - |
ATP | mutant enzyme T208D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 0.0387 | - |
ATP | mutant enzyme E268D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 0.04537 | - |
ATP | mutant enzyme T208A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 0.04647 | - |
ATP | mutant enzyme E268A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 0.04704 | - |
ATP | mutant enzyme N210A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 0.07667 | - |
ATP | mutant enzyme N210D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.26 | Corynebacterium ammoniagenes | - |
- |
- |
2.7.7.2 | Corynebacterium ammoniagenes | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.2 | - |
Corynebacterium ammoniagenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.26 | ATP + riboflavin | - |
Corynebacterium ammoniagenes | ADP + FMN | - |
? | |
2.7.7.2 | ATP + FMN | - |
Corynebacterium ammoniagenes | diphosphate + FAD | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.26 | ATP: riboflavin kinase | - |
Corynebacterium ammoniagenes |
2.7.1.26 | RFK | - |
Corynebacterium ammoniagenes |
2.7.1.26 | riboflavin kinase/FMN adenylyltransferase | FADS, bifunctional enzyme, the C-terminus exhibits ATP: riboflavin kinase activity | Corynebacterium ammoniagenes |
2.7.7.2 | ATP:FMN adenylyltransferase | - |
Corynebacterium ammoniagenes |
2.7.7.2 | FADS | - |
Corynebacterium ammoniagenes |
2.7.7.2 | FMNAT | - |
Corynebacterium ammoniagenes |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.26 | 0.042 | - |
ATP | apparent value, mutant enzyme N210D, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 0.045 | - |
riboflavin | apparent value, mutant enzyme E268D, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 0.07 | - |
ATP | apparent value, mutant enzyme E268D, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 0.085 | - |
riboflavin | apparent value, mutant enzyme N210D, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 1.13 | - |
ATP | apparent value, wild type enzyme, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 5 | - |
riboflavin | apparent value, wild type enzyme, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.26 | 0.83 | - |
ATP | apparent value, mutant enzyme N210D, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 3.83 | - |
ATP | apparent value, mutant enzyme E268D, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 10.83 | - |
riboflavin | apparent value, mutant enzyme E268D, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 40.3 | - |
riboflavin | apparent value, mutant enzyme N210D, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 82.17 | - |
ATP | apparent value, wild type enzyme, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.1.26 | 386 | - |
riboflavin | apparent value, wild type enzyme, at 37°C in 20 mM PIPES pH 7.0, 0.8 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 3.8 | - |
ATP | mutant enzyme N210D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 4.3 | - |
ATP | mutant enzyme N210A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 7.3 | - |
ATP | mutant enzyme E268A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 7.7 | - |
ATP | mutant enzyme T208A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 8 | - |
ATP | wild type enzyme, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 8.2 | - |
ATP | mutant enzyme E268D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 8.5 | - |
ATP | mutant enzyme T208D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 19.7 | - |
FMN | mutant enzyme N210D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 24.8 | - |
FMN | mutant enzyme N210A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 238.3 | - |
FMN | wild type enzyme, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 270.2 | - |
FMN | mutant enzyme E268D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 365.7 | - |
FMN | mutant enzyme T208D, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 369.2 | - |
FMN | mutant enzyme T208A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes | |
2.7.7.2 | 386 | - |
FMN | mutant enzyme E268A, at 37°C in 20 mM PIPES pH 7.0, 10 mM MgCl2 | Corynebacterium ammoniagenes |