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Literature summary extracted from

  • Han, S.; Jin, B.; Lee, W.; Yu, Y.
    Kinetic properties of wild-type and C117D mutant UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from Haemophilus influenzae (2011), Bull. Korean Chem. Soc., 32, 2549-2552.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.7 C117D the mutation shifts ithe optimum pH from 7.8 to 6.0. The mutant is not inhibited by 1 mM fosfomycin Haemophilus influenzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.7 fosfomycin more than 90% inhibition at 0.2 mM Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.7 0.024
-
 phosphoenolpyruvate wild type enzyme, at pH 7.8 and 25°C Haemophilus influenzae
2.5.1.7 0.031
-
 UDP-N-acetyl-D-glucosamine wild type enzyme, at pH 7.8 and 25°C Haemophilus influenzae
2.5.1.7 0.15
-
 phosphoenolpyruvate mutant enzyme C117D, at pH 6.0 and 25°C Haemophilus influenzae
2.5.1.7 0.16
-
 UDP-N-acetyl-D-glucosamine mutant enzyme C117D, at pH 6.0 and 25°C Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.7 Haemophilus influenzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.7 Ni-NTA column chromatography and gel filtration Haemophilus influenzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.7 0.91
-
 mutant enzyme C117D, at pH 6.0 and 25°C Haemophilus influenzae
2.5.1.7 4.7
-
 wild type enzyme, at pH 7.8 and 25°C Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Haemophilus influenzae UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.7 MurA
-
 Haemophilus influenzae
2.5.1.7 UDP-N-acetylglucosamine enolpyruvyl transferase
-
 Haemophilus influenzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.7 0.68
-
 phosphoenolpyruvate mutant enzyme C117D, at pH 6.0 and 25°C Haemophilus influenzae
2.5.1.7 0.68
-
 UDP-N-acetyl-D-glucosamine mutant enzyme C117D, at pH 6.0 and 25°C Haemophilus influenzae
2.5.1.7 3.5
-
 phosphoenolpyruvate wild type enzyme, at pH 7.8 and 25°C Haemophilus influenzae
2.5.1.7 3.5
-
 UDP-N-acetyl-D-glucosamine wild type enzyme, at pH 7.8 and 25°C Haemophilus influenzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.7 7.8
-
-
 Haemophilus influenzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.7 5.5 10 recombinant enzyme, enzyme activity gradually decreases when the pH is below 5.5 or above 10.0 Haemophilus influenzae