Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Koh, J.-U.; Chung, H.-J.; Chang, W.-Y.; Tanokura, M.; Kong, K.-H.
    Discovery and characterization of a thermostable NADH oxidase from Pyrococcus horikoshii OT3 (2009), Bull. Korean Chem. Soc., 30, 2984-2988.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.6.3.3 synthesis the enzyme is applicable as a biocatalyst for NAD+ recycling Pyrococcus horikoshii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.3.3 overexpressed in Escherichia coli Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.3.3 0.0037
-
 beta-NADH pH and temperature not specified in the publication Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.3.3 22000
-
 4 * 22000, SDS-PAGE Pyrococcus horikoshii
1.6.3.3 84000
-
 gel filtration Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.3.3 beta-NADH + H+ + O2 Pyrococcus horikoshii
-
 beta-NAD+ + H2O2
-
 ?
1.6.3.3 beta-NADH + H+ + O2 Pyrococcus horikoshii OT-3
-
 beta-NAD+ + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.3.3 Pyrococcus horikoshii O58049
-
-
1.6.3.3 Pyrococcus horikoshii OT-3 O58049
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.3.3
-
 Pyrococcus horikoshii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.3.3 84
-
 pH and temperature not specified in the publication Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.3.3 beta-NADH + H+ + O2
-
 Pyrococcus horikoshii beta-NAD+ + H2O2
-
 ?
1.6.3.3 beta-NADH + H+ + O2 the enzyme is specific for beta-NADH. No activity with alpha-NADH, alpha-NADPH, or beta-NADPH Pyrococcus horikoshii beta-NAD+ + H2O2
-
 ?
1.6.3.3 beta-NADH + H+ + O2
-
 Pyrococcus horikoshii OT-3 beta-NAD+ + H2O2
-
 ?
1.6.3.3 beta-NADH + H+ + O2 the enzyme is specific for beta-NADH. No activity with alpha-NADH, alpha-NADPH, or beta-NADPH Pyrococcus horikoshii OT-3 beta-NAD+ + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.3.3 homotetramer 4 * 22000, SDS-PAGE Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
1.6.3.3 PH0311
-
 Pyrococcus horikoshii
1.6.3.3 PhNOX
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.3.3 95
-
-
 Pyrococcus horikoshii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.6.3.3 55 100 55°C: about 60% of maximal activity, 100°C: about 80% of maximal activity Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.3.3 90
-
 half-life: 5 h Pyrococcus horikoshii
1.6.3.3 95
-
 half-life: 4 h Pyrococcus horikoshii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.3.3 115
-
 beta-NADH pH and temperature not specified in the publication Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.3.3 8
-
-
 Pyrococcus horikoshii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.3.3 6 9.5 pH 6.0: about 60% of maximal activity, pH 9.5: about 60% of maximal activity Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.3.3 FAD FAD-dependent enzyme Pyrococcus horikoshii