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Literature summary extracted from

  • Yeh, C.; Hsieh, L.; Yang, C.; Lee, P.
    Molecular characterization of isopentenyltransferase (BoAIPT1) from Bambusa oldhamii expressed in Escherichia coli (2011), Bot. Stud., 52, 249-256.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.27 cDNA encoding BoAIPT1, DNA and amino acid sequence determination and analysis, sequence comparison, functional expression of N-terminally His6-tagged BoAIPT1 in Escherichia coli strain BL21 (DE3), optimum protein expression is obtained by incubating the IPTG-induced culture at 18°C for 18 hr, transgenic expression in bamboo plants, real-time PCR expression analysis Bambusa oldhamii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.27 Mg2+ required Bambusa oldhamii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.27 40000
-
 x * 40000, recombinant His6-tagged BoAIPT1, SDS-PAGE Bambusa oldhamii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.27 dimethylallyl diphosphate + AMP Bambusa oldhamii
-
 diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.27 Bambusa oldhamii D7R812
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.27 recombinant His6-tagged BoAIPT1 from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and ultrafiltration Bambusa oldhamii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.27 shoot
-
 Bambusa oldhamii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.27 dimethylallyl diphosphate + AMP
-
 Bambusa oldhamii diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.27 ? x * 40000, recombinant His6-tagged BoAIPT1, SDS-PAGE Bambusa oldhamii

Synonyms

EC Number Synonyms Comment Organism
2.5.1.27 adenylate isopentenyltransferase
-
 Bambusa oldhamii
2.5.1.27 AIPT1
-
 Bambusa oldhamii
2.5.1.27 isopentenyltransferase
-
 Bambusa oldhamii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.27 30
-
 assay at Bambusa oldhamii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.27 7.5
-
 assay at Bambusa oldhamii

General Information

EC Number General Information Comment Organism
2.5.1.27 additional information transgenic expression of BoAIPT1 in bamboo plants increases the green bamboo shoot growth, with the top green shoot section having the highest expression level Bambusa oldhamii