Literature summary extracted from

Cardenas-Fernandez, M.; Neto, W.; Lopez, C.; Alvaro, G.; Tufvesson, P.; Woodley, J.M.
Immobilization of Escherichia coli containing omega-transaminase activity in LentiKats (2012), Biotechnol. Prog., 28, 693-698.

Application

EC Number	Application	Comment	Organism
2.6.1.18	biotechnology	recombinant Escherichia coli cells containing overexpressed omega-TA activity are immobilized by entrapment in LentiKats and the effect of permeabilization on free cells and their immobilized counterparts is analyzed. Cells are applied in the synthesis of the aromatic amines 3-amino-1-phenylbutane and 1-phenylethylamine. The best results are obtained with CTAB 0.1% for permeabilization which results in an increase in reaction rate by 40% compared to the whole cells. The synthesis of 1-phenylethylamine is carried out using isopropyl amine and L-alanine as amino donors. Using whole cell biocatalysis, the reaction with isopropyl amine is one order of magnitude faster than with L-alanine	Escherichia coli

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.6.1.18	expressed in Escherichia coli	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.6.1.18	Escherichia coli	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.6.1.18	1-phenylethylamine + pyruvate	-	Escherichia coli	acetophenone + L-alanine	-	r
2.6.1.18	4-phenyl-2-butanone + isopropylamine	-	Escherichia coli	3-amino-1-phenylbutane + acetone	-	ir

Synonyms

EC Number	Synonyms	Comment	Organism
2.6.1.18	omega-transaminase	-	Escherichia coli

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.6.1.18	30	-	assay at	Escherichia coli

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.6.1.18	7	-	assay at	Escherichia coli

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
2.6.1.18	pyridoxal 5'-phosphate	-	Escherichia coli

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Release 2023.1 (January 2023)