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Literature summary extracted from

  • Van der Borght, J.; Soetaert, W.; Desmet, T.
    Engineering the acceptor specificity of trehalose phosphorylase for the production of trehalose analogs (2012), Biotechnol. Prog., 28, 1257-1262.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.64 expressed in Escherichia coli BL21(DE3) cells Thermoanaerobacter brockii

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.64 A431T the mutant shows strongly decreased catalytic efficiency for D-glucose and decreased catalytic efficiency for D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 A440E the mutant shows increased catalytic efficiency for D-glucose and wild type catalytic efficiency for D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 A440K the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 A440M the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 A440V the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 A440V/N657Y the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 A440V/R448S the mutant shows increased catalytic efficiency for D-glucose and strongly increased catalytic efficiency for D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 N657D the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 N657G the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 N657I the mutant shows approximate 2fold increase in affinity for D-galactose Thermoanaerobacter brockii
2.4.1.64 N657L the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 N657R the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 N657Y the mutant shows approximate 2fold increase in affinity for D-galactose Thermoanaerobacter brockii
2.4.1.64 P588H the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 R448D the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 R448F the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 R448N the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 R448S the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme Thermoanaerobacter brockii
2.4.1.64 R448S/N657Y the mutant shows about wild type catalytic efficiencies for D-glucose and D-galactose Thermoanaerobacter brockii
2.4.1.64 R448V the mutant shows increased catalytic efficiencies for D-glucose and D-galactose compared to the wild type enzyme Thermoanaerobacter brockii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.64 0.6
-
 D-glucose mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.6
-
 D-glucose mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.7
-
 D-glucose mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 1.2
-
 D-glucose mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 1.2
-
 D-glucose mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 1.3
-
 D-glucose mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 1.5
-
 D-glucose mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 1.5
-
 D-glucose mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 1.5
-
 D-glucose mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 1.8
-
 D-glucose mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 1.9
-
 D-glucose mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 1.9
-
 D-glucose mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 2.1
-
 D-glucose mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 2.1
-
 D-glucose mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 2.2
-
 D-glucose mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 2.3
-
 D-glucose mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 2.3
-
 D-glucose mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 2.5
-
 D-glucose mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 3
-
 D-glucose mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 3 3.5 D-galactose mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 3.2
-
 D-glucose wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 30.1
-
 D-galactose mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 35.1
-
 D-galactose mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 36.8
-
 D-glucose mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 38
-
 D-galactose mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 43.7
-
 D-galactose mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 43.8
-
 D-galactose mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 45.2
-
 D-galactose mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 45.7
-
 D-galactose mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 45.8
-
 D-galactose mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 47.5
-
 D-galactose mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 53.5
-
 D-galactose mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 53.6
-
 D-galactose mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 56.8
-
 D-galactose mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 58.1
-
 D-galactose mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 61.1
-
 D-galactose mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 61.9
-
 D-galactose mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 62.5
-
 D-galactose mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 68.2
-
 D-galactose mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 96
-
 D-galactose mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 98
-
 D-galactose wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 447.6
-
 D-galactose mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.64 Thermoanaerobacter brockii Q8L164 previously known as Thermoanaerobium brockii
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.64
-
 Thermoanaerobacter brockii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.64 beta-D-glucose 1-phosphate + D-galactose low activity. The Km value for D-galactose is about 30fold higher than that for D-glucose Thermoanaerobacter brockii ? + phosphate
-
 r
2.4.1.64 D-glucose + beta-D-glucose 1-phosphate best substrate combination Thermoanaerobacter brockii alpha,alpha-trehalose + phosphate
-
 r
2.4.1.64 additional information the wild type enzyme shows low activity with D-fructose and intermediate activity with D-mannose Thermoanaerobacter brockii ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.64 trehalose phosphorylase
-
 Thermoanaerobacter brockii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.64 60
-
 the half-life of the wild type enzyme remains constant for about 13 h at 60°C Thermoanaerobacter brockii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.64 7
-
 D-glucose mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 14
-
 D-galactose mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 16.1
-
 D-glucose mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 17
-
 D-galactose mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 18.6
-
 D-glucose mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 21.1
-
 D-galactose mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 21.7
-
 D-glucose mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 22.6
-
 D-galactose mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 23
-
 D-galactose mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 23.3
-
 D-galactose mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 23.6
-
 D-galactose mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 24
-
 D-galactose mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 25
-
 D-glucose mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 26.2
-
 D-galactose mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 26.3
-
 D-glucose mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 27.4
-
 D-glucose mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 27.7
-
 D-galactose mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 30.6
-
 D-glucose mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 31.4
-
 D-glucose mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 31.9
-
 D-galactose mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 32.1
-
 D-glucose mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 32.4
-
 D-galactose mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 32.7
-
 D-glucose mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 32.9
-
 D-galactose mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 32.9
-
 D-galactose wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 33.3
-
 D-galactose mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 33.9
-
 D-glucose wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 35.5
-
 D-galactose mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 36.1
-
 D-glucose mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 38.3
-
 D-galactose mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 38.9
-
 D-galactose mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 41.2
-
 D-glucose mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 42
-
 D-glucose mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 42.2
-
 D-galactose mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 42.7
-
 D-glucose mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 43.3
-
 D-glucose mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 43.5
-
 D-glucose mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 46.9
-
 D-glucose mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 51.2
-
 D-glucose mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 53.9
-
 D-glucose mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 54.3
-
 D-galactose mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 66.5
-
 D-galactose mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.64 0.149
-
 D-galactose mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.336
-
 D-galactose wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.337
-
 D-galactose mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.368
-
 D-galactose mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.388
-
 D-galactose mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.413
-
 D-galactose mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.444
-
 D-galactose mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.488
-
 D-galactose mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.5
-
 D-galactose mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.509
-
 D-galactose mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.545
-
 D-galactose mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.566
-
 D-galactose mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.574
-
 D-galactose mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.6
-
 D-galactose mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.604
-
 D-galactose mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.613
-
 D-galactose mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.672
-
 D-galactose mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.72
-
 D-galactose mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.789
-
 D-galactose mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.872
-
 D-glucose mutant enzyme A431T, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 0.952
-
 D-galactose mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 1.29
-
 D-galactose mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 11
-
 D-glucose wild type enzyme, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 12
-
 D-glucose mutant enzyme R448S/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 13
-
 D-glucose mutant enzyme A440M, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 14
-
 D-glucose mutant enzyme A440E, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 16
-
 D-glucose mutant enzyme R448D, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 17
-
 D-glucose mutant enzyme N657G, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 18
-
 D-glucose mutant enzyme A440K, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 18
-
 D-glucose mutant enzyme N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 18
-
 D-glucose mutant enzyme R448F, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 18
-
 D-glucose mutant enzyme R448S, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 19
-
 D-glucose mutant enzyme R448V, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 20
-
 D-glucose mutant enzyme N657L, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 20
-
 D-glucose mutant enzyme R448N, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 22
-
 D-glucose mutant enzyme A440V, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 22
-
 D-glucose mutant enzyme N657I, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 24
-
 D-glucose mutant enzyme N657R, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 27
-
 D-glucose mutant enzyme A440V/N657Y, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 28
-
 D-glucose mutant enzyme N657D, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 29
-
 D-glucose mutant enzyme P588H, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii
2.4.1.64 36
-
 D-glucose mutant enzyme A440V/R448S, in 50 mM MES buffer at pH 6.0 and 60°C Thermoanaerobacter brockii