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Literature summary extracted from

  • Agrawal, M.; Chen, R.R.
    Discovery and characterization of a xylose reductase from Zymomonas mobilis ZM4 (2011), Biotechnol. Lett., 33, 2127-2133.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.431 gene ZMO0976, expression of His-tagged enzyme in Escherichia coli strain UT5600 Zymomonas mobilis subsp. mobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.431 additional information
-
 additional information Michaelis-Menten kinetics Zymomonas mobilis subsp. mobilis
1.1.1.431 1.8
-
 benzaldehyde pH 7.2, temperature not specified in the publication Zymomonas mobilis subsp. mobilis
1.1.1.431 4.2
-
 furfural pH 7.2, temperature not specified in the publication Zymomonas mobilis subsp. mobilis
1.1.1.431 258
-
 D-xylose pH 7.2, temperature not specified in the publication Zymomonas mobilis subsp. mobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.431 D-xylose + NADH + H+ Zymomonas mobilis subsp. mobilis
-
 xylitol + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.431 Zymomonas mobilis subsp. mobilis F2YCN5 ssp. mobilis, and strain A3, an engineered strain ZM4 that is adapted to 5% D-xylose, gene ZMO0976
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.431 recombinant His-tagged enzyme from Escherichia coli strain UT5600 by nickel affinity chromatography Zymomonas mobilis subsp. mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.431 5-hydroxymethylfurfural + NADH + H+ low activity Zymomonas mobilis subsp. mobilis (furan-2,5-diyl)dimethanol + NAD+
-
 r
1.1.1.431 acetaldehyde + NADH + H+
-
 Zymomonas mobilis subsp. mobilis ethanol + NAD+
-
 r
1.1.1.431 benzaldehyde + NADH + H+ best substrate Zymomonas mobilis subsp. mobilis benzyl alcohol + NAD+
-
 r
1.1.1.431 D-xylose + NADH + H+
-
 Zymomonas mobilis subsp. mobilis xylitol + NAD+
-
 r
1.1.1.431 furfural + NADH + H+
-
 Zymomonas mobilis subsp. mobilis (furan-2-yl)methanol + NAD+
-
 r
1.1.1.431 xylulose + NADH + H+
-
 Zymomonas mobilis subsp. mobilis ? + NAD+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.431 xylose reductase
-
 Zymomonas mobilis subsp. mobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.431 7.2
-
 assay at Zymomonas mobilis subsp. mobilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.431 NAD+
-
 Zymomonas mobilis subsp. mobilis
1.1.1.431 NADH
-
 Zymomonas mobilis subsp. mobilis