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Literature summary extracted from
- Purification and gene cloning of a dehydrogenase from Lactobacillus brevis that catalyzes a reaction involved in aflatoxin biosynthesis (2008), Biosci. Biotechnol. Biochem., 72, 724-734.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.352 | - |
Levilactobacillus brevis |
| 1.2.1.B8 | - |
Levilactobacillus brevis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.352 | 0.0426 | - |
5'-hydroxyaverantin | in 30 mM potassium phosphate buffer (pH 7.5), at 37°C | Levilactobacillus brevis |  |
| 1.2.1.B8 | 0.0426 | - |
5'-hydroxyaverantin | pH 7.5, 37°C | Levilactobacillus brevis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.352 | 25873 | - |
3 * 25873, calculated from amino acid sequence | Levilactobacillus brevis |
| 1.1.1.352 | 25918 | - |
3 * 25918 MALDI TOF mass spectrometry | Levilactobacillus brevis |
| 1.1.1.352 | 33000 | - |
3 * 33000, SDS-PAGE | Levilactobacillus brevis |
| 1.1.1.352 | 100000 | - |
gel filtration | Levilactobacillus brevis |
| 1.2.1.B8 | 25873 | - |
x * 25873, calculated from sequence, TOF MS analysis | Levilactobacillus brevis |
| 1.2.1.B8 | 33000 | - |
x * 33000, SDS-PAGE | Levilactobacillus brevis |
| 1.2.1.B8 | 100000 | - |
gel filtration | Levilactobacillus brevis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.352 | 5'-hydroxyaverantin + NADP+ | Levilactobacillus brevis | - |
5'-oxoaverantin + NADPH + H+ | - |
ir | |
| 1.1.1.352 | 5'-hydroxyaverantin + NADP+ | Levilactobacillus brevis IFO 12005 | - |
5'-oxoaverantin + NADPH + H+ | - |
ir | |
| 1.2.1.B8 | 5'-hydroxyaverantin + NADP+ | Levilactobacillus brevis | the enzyme catalyzes a reaction in aflatoxin biosynthesis | (1'S)-5'-oxoaverantin + NADPH + H+ | - |
? | |
| 1.2.1.B8 | 5'-hydroxyaverantin + NADP+ | Levilactobacillus brevis IFO 12005 | the enzyme catalyzes a reaction in aflatoxin biosynthesis | (1'S)-5'-oxoaverantin + NADPH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.352 | Levilactobacillus brevis | - |
- |
- |
| 1.1.1.352 | Levilactobacillus brevis IFO 12005 | - |
- |
- |
| 1.2.1.B8 | Levilactobacillus brevis | - |
- |
- |
| 1.2.1.B8 | Levilactobacillus brevis IFO 12005 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.352 | DEAE Sepharose CL-6B column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, and Sephacryl S-300 gel filtration | Levilactobacillus brevis |
| 1.2.1.B8 | - |
Levilactobacillus brevis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.352 | 0.00613 | - |
cell extract, at pH 7.5 and 37°C | Levilactobacillus brevis |
| 1.1.1.352 | 0.552 | - |
after 90fold purification, at pH 7.5 and 37°C | Levilactobacillus brevis |
| 1.2.1.B8 | 0.552 | - |
pH 7.5, 37°C | Levilactobacillus brevis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.352 | 5'-hydroxyaverantin + NADP+ | - |
Levilactobacillus brevis | 5'-oxoaverantin + NADPH + H+ | - |
ir | |
| 1.1.1.352 | 5'-hydroxyaverantin + NADP+ | - |
Levilactobacillus brevis IFO 12005 | 5'-oxoaverantin + NADPH + H+ | - |
ir | |
| 1.1.1.352 | additional information | no activity with NAD+ | Levilactobacillus brevis | ? | - |
? | |
| 1.1.1.352 | additional information | no activity with NAD+ | Levilactobacillus brevis IFO 12005 | ? | - |
? | |
| 1.2.1.B8 | 5'-hydroxyaverantin + NADP+ | the enzyme catalyzes a reaction in aflatoxin biosynthesis | Levilactobacillus brevis | (1'S)-5'-oxoaverantin + NADPH + H+ | - |
? | |
| 1.2.1.B8 | 5'-hydroxyaverantin + NADP+ | a mixture of two diastereomers, (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin | Levilactobacillus brevis | (1'S)-5'-oxoaverantin + NADPH + H+ | - |
? | |
| 1.2.1.B8 | 5'-hydroxyaverantin + NADP+ | the enzyme catalyzes a reaction in aflatoxin biosynthesis | Levilactobacillus brevis IFO 12005 | (1'S)-5'-oxoaverantin + NADPH + H+ | - |
? | |
| 1.2.1.B8 | 5'-hydroxyaverantin + NADP+ | a mixture of two diastereomers, (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin | Levilactobacillus brevis IFO 12005 | (1'S)-5'-oxoaverantin + NADPH + H+ | - |
? | |
| 1.2.1.B8 | additional information | no activity with nidurufin, averantin, and 6-phenylhexal-1-ol | Levilactobacillus brevis | ? | - |
? | |
| 1.2.1.B8 | additional information | no activity with nidurufin, averantin, and 6-phenylhexal-1-ol | Levilactobacillus brevis IFO 12005 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.352 | homotrimer | 3 * 33000, SDS-PAGE | Levilactobacillus brevis |
| 1.1.1.352 | homotrimer | 3 * 25873, calculated from amino acid sequence | Levilactobacillus brevis |
| 1.1.1.352 | homotrimer | 3 * 25918 MALDI TOF mass spectrometry | Levilactobacillus brevis |
| 1.2.1.B8 | ? | x * 33000, SDS-PAGE | Levilactobacillus brevis |
| 1.2.1.B8 | ? | x * 25873, calculated from sequence, TOF MS analysis | Levilactobacillus brevis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.352 | alcohol dehydrogenase | - |
Levilactobacillus brevis |
| 1.1.1.352 | HAVN dehydrogenase | - |
Levilactobacillus brevis |
| 1.2.1.B8 | Lac-HAVN dehydrogenase | - |
Levilactobacillus brevis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.352 | 40 | 55 | - |
Levilactobacillus brevis |
| 1.2.1.B8 | 37 | - |
assay at | Levilactobacillus brevis |
| 1.2.1.B8 | 40.55 | - |
- |
Levilactobacillus brevis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.B8 | 35 | 60 | 35°C: about 65% of maximal activity, 60°C: about 30% of maximal activity | Levilactobacillus brevis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.352 | 7 | 8 | no activity is detected above pH 9.0 | Levilactobacillus brevis |
| 1.2.1.B8 | 7 | 8 | - |
Levilactobacillus brevis |
| 1.2.1.B8 | 7.5 | - |
assay at | Levilactobacillus brevis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.B8 | 6.5 | 8.5 | pH 6.5: about 75% of maximal activity, pH 8.5: about 30% of maximal activity | Levilactobacillus brevis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.352 | NADP+ | no activity with NAD+ | Levilactobacillus brevis | |
| 1.2.1.B8 | NADP+ | no activity with NAD+ | Levilactobacillus brevis | |
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