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Literature summary extracted from

  • Yeon, Y.J.; Park, H.Y.; Yoo, Y.J.
    Enzymatic reduction of levulinic acid by engineering the substrate specificity of 3-hydroxybutyrate dehydrogenase (2013), Biores. Technol., 134, 377-380.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.30 synthesis the engineered enzyme mutant H144L/W187F is used for production of 4-hydroxyvaleric acid, a monomer of bio-polyester and a precursor of bio-fuels, from levulinic acid Alcaligenes faecalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.30 expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) Alcaligenes faecalis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.30 H144L/W187F site-directed mutagenesis, the mutant shows activity with levulinnic acid, in contrast to the wild-type enzyme, and is engineered for production of 4-hydroxyvaleric acid, molecular docking simulation, overview Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+ Alcaligenes faecalis
-
 acetoacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.30 Alcaligenes faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.30 recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Alcaligenes faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Alcaligenes faecalis acetoacetate + NADH + H+
-
 r
1.1.1.30 additional information no activity of the wild-type enzyme with levulinic acid, enzyme mutant H144L/W187F is active with levulinic acid producing 4-hydroxyvaleric acid Alcaligenes faecalis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.30 3HBDH
-
 Alcaligenes faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.30 30
-
 reduction reaction Alcaligenes faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.30 6
-
 reduction reaction Alcaligenes faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.30 NAD+
-
 Alcaligenes faecalis

General Information

EC Number General Information Comment Organism
1.1.1.30 additional information Gln94, His144, Lys152, and Gln196 form hydrogen bonds with carboxyl and/or ketone group of acetoacetate, Trp187, Trp257 form hydrophobic interactions with the carbon atoms of acetoacetate, and Ser142 and Tyr155 are directly related to the catalytic mechanism Alcaligenes faecalis