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Literature summary extracted from

  • Chen, K.; Dou, J.; Tang, S.; Yang, Y.; Wang, H.; Fang, H.; Zhou, C.
    Deletion of the aroK gene is essential for high shikimic acid accumulation through the shikimate pathway in E. coli (2012), Biores. Technol., 119, 141-147.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.71 synthesis the aroK enzyme knockout strain of Escherichia coli is useful for shikimate production Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.71 generation of knockout and antisense strain from Escherichia coli parent strain DH5alpha Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.71 additional information inactivation of the aroK gene in an shikimate-producing Escherichia coli strain DHPYA-T7. In this strain, the aroL, ptsHIcrr and ydiB genes are deleted, and the tktA, glk, aroE and aroB genes are overexpressed. Accumulation of shikimate increases 2.69fold after aroK gene deletion and 1.29fold after antisense RNA interference. The activity of shikimate kinase in the knockout strain DHPYAAS-T7 is 0.21fold of that in the antisense strain DHPYAS-T7, while the accumulation of shikimate is 1.5fold in the knockout strain DHPYAAS-T7 compared to that in the antisense strain DHPYAS-T7 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.71 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.71 ATP + shikimate Escherichia coli
-
 ADP + 3-phosphoshikimate
-
 ?
2.7.1.71 ATP + shikimate Escherichia coli DHPYA-T7
-
 ADP + 3-phosphoshikimate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.71 Escherichia coli
-
 gene aroK
-
2.7.1.71 Escherichia coli DHPYA-T7
-
 gene aroK
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.71 ATP + shikimate
-
 Escherichia coli ADP + 3-phosphoshikimate
-
 ?
2.7.1.71 ATP + shikimate
-
 Escherichia coli DHPYA-T7 ADP + 3-phosphoshikimate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.71 9
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.71 ATP
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.7.1.71 malfunction aroK gene inactivation in DHPYA-T7 leads to high shikimate accumulation, especially when this inactivation is caused by chromosomal deletion Escherichia coli