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Literature summary extracted from

  • Takeda, K.; Sato, J.; Goto, K.; Fujita, T.; Watanabe, T.; Abo, M.; Yoshimura, E.; Nakagawa, J.; Abe, A.; Kawasaki, S.; Niimura, Y.
    Escherichia coli ferredoxin-NADP+ reductase and oxygen-insensitive nitroreductase are capable of functioning as ferric reductase and of driving the Fenton reaction (2010), Biometals, 23, 727-737.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 expressed in Escherichia coli JM109 cells Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.0018
-
 NADPH in the presence of 0.15 mM Fe(III)-EDTA, in 50 mM sodium phosphate (pH 7.0), at 25°C Escherichia coli
1.18.1.2 0.00364
-
 Fe(III)-EDTA in the presence of 0.15 mM Fe(III)-EDTA, in the presence of 0.15 mM FAD, in 50 mM sodium phosphate (pH 7.0), at 25°C Escherichia coli
1.18.1.2 0.0396
-
 Fe(III)-EDTA in the presence of 0.15 mM Fe(III)-EDTA, in the absence of free flavin, in 50 mM sodium phosphate (pH 7.0), at 25°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 28000
-
 x * 28000, SDS-PAGE Escherichia coli
1.18.1.2 28981
-
 x * 28981, calculated from amino acid sequence Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Escherichia coli P28861
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2 butyl Toyopearl column chromatography and DEAE Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 2 ferricyanide + NADPH best substrate Escherichia coli 2 ferrocyanide + NADP+ + H+
-
 ?
1.18.1.2 cytochrome c + NADPH + H+ weakest substrate Escherichia coli ?
-
 ?
1.18.1.2 Fe(III)-deferoxamine + NADPH + H+
-
 Escherichia coli ?
-
 ?
1.18.1.2 Fe(III)-diethylenetriamine-N,N,N',N'',N''-pentaacetic acid + NADPH + H+ highest activity Escherichia coli Fe(II) + diethylenetriamine-N,N,N',N'',N''-pentaacetic acid + NADP+
-
 ?
1.18.1.2 Fe(III)-EDTA + NADPH + H+
-
 Escherichia coli Fe(II) + EDTA + NADP+
-
 ?
1.18.1.2 Fe(III)-ferrichrome + NADPH + H+
-
 Escherichia coli ?
-
 ?
1.18.1.2 Fe(III)-nitrilotriacetic acid + NADPH + H+
-
 Escherichia coli Fe(II) + nitrilotriacetic acid + NADP+
-
 ?
1.18.1.2 ferric citrate + NADPH + H+
-
 Escherichia coli ?
-
 ?
1.18.1.2 ferric enterobactin + NADPH + H+ lowest activity Escherichia coli ?
-
 ?
1.18.1.2 oxidized 2,6-dichlorophenolindophenol + NADPH + H+
-
 Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.1.2 ? x * 28000, SDS-PAGE Escherichia coli
1.18.1.2 ? x * 28981, calculated from amino acid sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin-NADP+ reductase
-
 Escherichia coli
1.18.1.2 ferric reductase
-
 Escherichia coli
1.18.1.2 FPR
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 3
-
 Fe(III)-EDTA in the presence of 0.15 mM Fe(III)-EDTA, in the absence of free flavin, in 50 mM sodium phosphate (pH 7.0), at 25°C Escherichia coli
1.18.1.2 3.53
-
 Fe(III)-EDTA in the presence of 0.15 mM Fe(III)-EDTA, in the presence of 0.15 mM FAD, in 50 mM sodium phosphate (pH 7.0), at 25°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 FAD contains FAD, the Km for FAD is 0.0429 mM Escherichia coli
1.18.1.2 NADPH
-
 Escherichia coli

Expression

EC Number Organism Comment Expression
1.18.1.2 Escherichia coli the level of Fpr is significantly increased in Escherichia coli cultured in lower iron concentrations (0.017-12.2 ppm) up

General Information

EC Number General Information Comment Organism
1.18.1.2 metabolism the enzyme also drives the Fenton reaction Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.18.1.2 76.3
-
 Fe(III)-EDTA in the presence of 0.15 mM Fe(III)-EDTA, in the absence of free flavin, in 50 mM sodium phosphate (pH 7.0), at 25°C Escherichia coli
1.18.1.2 972
-
 Fe(III)-EDTA in the presence of 0.15 mM Fe(III)-EDTA, in the presence of 0.15 mM FAD, in 50 mM sodium phosphate (pH 7.0), at 25°C Escherichia coli