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Literature summary extracted from

  • Guenther Sillero, M.A.; de Diego, A.; Sillero, A.
    Methylenebisphosphonate and triphosphate derivatives of the mevalonate pathway are substrates of yeast UTP:glucose-1-phosphate uridylyltransferase (2012), Biochimie, 94, 1871-1875.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 0.21
-
 geranyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
2.7.7.9 0.32
-
 farnesyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
2.7.7.9 0.51
-
 isopentenyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
2.7.7.9 1.1
-
 methylenebisphosphonate pH 8.0, 30°C Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.9 Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.9 UTP + alpha-D-glucose 1-phosphate Saccharomyces cerevisiae
-
 diphosphate + UDP-glucose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Saccharomyces cerevisiae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.9 commercial preparation
-
 Saccharomyces cerevisiae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 farnesyl triphosphate + UDP-glucose
-
 Saccharomyces cerevisiae farnesyl-tetraphosphouridine + alpha-D-glucose + diphosphate
-
 ?
2.7.7.9 geranyl triphosphate + UDP-glucose
-
 Saccharomyces cerevisiae geranyl-tetraphosphouridine + alpha-D-glucose + diphosphate
-
 ?
2.7.7.9 isopentenyl triphosphate + UDP-glucose
-
 Saccharomyces cerevisiae isopentenyl-tetraphosphouridine + alpha-D-glucose + diphosphate
-
 ?
2.7.7.9 methylenebisphosphonate + UDP-glucose
-
 Saccharomyces cerevisiae uridine 5'-(beta,gamma-methylenetriphosphate) + alpha-D-glucose + diphosphate
-
 ?
2.7.7.9 additional information the bisphosphonates alendronate, pamidronate, clodronate and etidronate are not acceptors of UMP. But UDP-glucose serves as uridylyl donor to triphosphate derivatives of the mevalonate pathway, farnesyl triphosphate, geranyl triphosphate, and isopentenyl triphosphate, with formation of farnesyl-tetraphosphouridine, geranyl-tetraphosphouridine, and isopentenyl-tetraphosphouridine, overview. No activity with farnesyl-, geranyl-, or isopentenyl-diphosphates or with dimethylallyl pyrophosphate Saccharomyces cerevisiae ?
-
 ?
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Saccharomyces cerevisiae diphosphate + UDP-glucose
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.9 UTP:glucose-1-phosphate uridylyltransferase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.9 30
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.9 0.013
-
 isopentenyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
2.7.7.9 0.033
-
 farnesyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
2.7.7.9 0.038
-
 geranyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
2.7.7.9 27
-
 methylenebisphosphonate pH 8.0, 30°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.9 8
-
 assay at Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.9 0.025
-
 isopentenyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
2.7.7.9 0.103
-
 farnesyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
2.7.7.9 0.181
-
 geranyl triphosphate pH 8.0, 30°C Saccharomyces cerevisiae
2.7.7.9 24.55
-
 methylenebisphosphonate pH 8.0, 30°C Saccharomyces cerevisiae