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Literature summary extracted from

  • Stranska, J.; Tylichova, M.; Kopecny, D.; Snegaroff, J.; Sebela, M.
    Biochemical characterization of pea ornithine-delta-aminotransferase: substrate specificity and inhibition by di- and polyamines (2010), Biochimie, 92, 940-948.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.13 expressed in Escherichia coli as a His-tagged fusion protein Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.13 1,3-diaminopropane
-
 Pisum sativum
2.6.1.13 diethylenetriamine
-
 Pisum sativum
2.6.1.13 L-norvaline
-
 Pisum sativum
2.6.1.13 norspermidine
-
 Pisum sativum
2.6.1.13 putrescine
-
 Pisum sativum
2.6.1.13 spermidine
-
 Pisum sativum
2.6.1.13 spermine
-
 Pisum sativum
2.6.1.13 triethylenetetramine
-
 Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.13 15
-
 L-ornithine pH 8.0, 37°C Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.13 50000
-
 monomer Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.13 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.13 using Ni-NTA chromatography Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.13 L-ornithine + 2-oxoglutarate
-
 Pisum sativum DELTA1-pyrroline-5-carboxylate + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.13 ornithine-delta-aminotransferase
-
 Pisum sativum
2.6.1.13 PsOAT
-
 Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.13 43
-
-
 Pisum sativum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.13 58
-
 temperature at which 50% of the activity is retained upon 30 min Pisum sativum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.13 4.3
-
 L-ornithine pH 8.0, 37°C Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.13 8.8
-
-
 Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.13 pyridoxal 5'-phosphate
-
 Pisum sativum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.13 0.8
-
 triethylenetetramine pH 8.0, 37°C Pisum sativum
2.6.1.13 2 5 spermidine pH 8.0, 37°C Pisum sativum
2.6.1.13 3
-
 diethylenetriamine pH 8.0, 37°C Pisum sativum
2.6.1.13 7
-
 L-norvaline pH 8.0, 37°C Pisum sativum
2.6.1.13 13
-
 norspermidine pH 8.0, 37°C Pisum sativum
2.6.1.13 17
-
 spermine pH 8.0, 37°C Pisum sativum
2.6.1.13 35
-
 1,3-diaminopropane pH 8.0, 37°C Pisum sativum
2.6.1.13 90
-
 putrescine pH 8.0, 37°C Pisum sativum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.13 0.2867
-
 L-ornithine pH 8.0, 37°C Pisum sativum