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Literature summary extracted from

  • Shell, J.R.; Lawrence, D.S.
    Probes of the mitochondrial cAMP-dependent protein kinase (2013), Biochim. Biophys. Acta, 1834, 1359-1363.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.11 additional information oligomycin, antimycin A, rotenone, CCCP, sodium azide, or Ca2+ activate calpain which activates the cAMP-dependent protein kinase Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.11 0.0019
-
 N-(8-([4-[3-(ethoxycarbonyl)-6,8,8-trimethyl-2-oxo-7,8-dihydro-2H-pyrano[3,2-g]quinolin-9(6H)-yl]butanoyl]amino)octanoyl)-GRTGRRFSYP-amide pH and temperature not specified in the publication Bos taurus
2.7.11.11 0.0022
-
 N-(8-([[7-(diethylamino)-2-oxo-2H-chromen-3-yl]carbonyl]amino)octanoyl)-GRTGRRFSYP-amide pH and temperature not specified in the publication Bos taurus
2.7.11.11 0.0062
-
 N-(8-[[(11-oxo-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-10-yl)carbonyl]amino]octanoyl)-GRTGRRFSYP-amide pH and temperature not specified in the publication Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.11 mitochondrion associated with the inner membrane/matrix Bos taurus 5739
-
2.7.11.11 additional information the enzyme is anchored to a variety of intracellular locations via interaction with A-kinase anchoring proteins Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.11 additional information Ca2+ activates calpain which activates the cAMP-dependent protein kinase Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.11 proteolytic modification the cAMP-dependent protein kinase is a serine/threonine kinase that exists as an inactive tetrameric holoenzyme consisting of two regulatory subunits and two catalytic subunits. It is activated by digestion of the regulatory subunits through calpain releasing the active catalytic ssubunits Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.11 heart
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.11 ATP + N-(8-([4-[3-(ethoxycarbonyl)-6,8,8-trimethyl-2-oxo-7,8-dihydro-2H-pyrano[3,2-g]quinolin-9(6H)-yl]butanoyl]amino)octanoyl)-GRTGRRFSYP-amide
-
 Bos taurus ADP + N-(8-([4-[3-(ethoxycarbonyl)-6,8,8-trimethyl-2-oxo-7,8-dihydro-2H-pyrano[3,2-g]quinolin-9(6H)-yl]butanoyl]amino)octanoyl)-GRTGRRFpSYP-amide
-
 ?
2.7.11.11 ATP + N-(8-([[7-(diethylamino)-2-oxo-2H-chromen-3-yl]carbonyl]amino)octanoyl)-GRTGRRFSYP-amide
-
 Bos taurus ADP + N-(8-([[7-(diethylamino)-2-oxo-2H-chromen-3-yl]carbonyl]amino)octanoyl)-GRTGRRFpSYP-amide
-
 ?
2.7.11.11 ATP + N-(8-[[(11-oxo-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-10-yl)carbonyl]amino]octanoyl)-GRTGRRFSYP-amide
-
 Bos taurus ADP + N-(8-[[(11-oxo-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-10-yl)carbonyl]amino]octanoyl)-GRTGRRFpSYP-amide
-
 ?
2.7.11.11 additional information development of kinase activity sensor fluorogenic substrates, upon phosphorylation the peptide interacts with a phosphoserine-binding protein 14-3-3 domain, displacing the quencher, and resulting in a burst of fluorescence, overview Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.11 dimer beta2, the cAMP-dependent protein kinase is a serine/threonine kinase that exists as an inactive tetrameric holoenzyme consisting of two regulatory subunits and two catalytic subunits. It is activated by digestion of the regulatory subunits through calpain releasing the active catalytic ssubunits Bos taurus
2.7.11.11 tetramer alpha2beta2, the cAMP-dependent protein kinase is a serine/threonine kinase that exists as an inactive tetrameric holoenzyme consisting of two regulatory subunits and two catalytic subunits. It is activated by digestion of the regulatory subunits through calpain Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 PKA
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.11 ATP
-
 Bos taurus

General Information

EC Number General Information Comment Organism
2.7.11.11 physiological function PKA activity at the mitochondria is associated with the regulation of apoptosis, mitochondrial respiration, and ATP synthesis. Mitochondrial cAMP-dependent protein kinase is regulted by the protease calpain. Upon exposure of bovine heart mitochondria to digitonin, Ca2+, and a variety of electron transport chain inhibitors, the regulatory subunits of the PKA holoenzyme are digested by calpain, releasing active catalytic subunits. The proteolysis can be attenuated by calpain inhibitor I Bos taurus