Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Suzuki, R.; Terasawa, K.; Kimura, K.; Fujimoto, Z.; Momma, M.; Kobayashi, M.; Kimura, A.; Funane, K.
    Biochemical characterization of a novel cycloisomaltooligosaccharide glucanotransferase from Paenibacillus sp. 598K (2012), Biochim. Biophys. Acta, 1824, 919-924.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.248 gene cit, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Paenibacillus sp.
2.4.1.248 gene cit, expression in Escherichia coli Niallia circulans

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.248 D144A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Paenibacillus sp.
2.4.1.248 D144N site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Paenibacillus sp.
2.4.1.248 D264A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Paenibacillus sp.
2.4.1.248 D269A site-directed mutagenesis, the mutant is inactive Paenibacillus sp.
2.4.1.248 D269N site-directed mutagenesis, the mutant is almost inactive Paenibacillus sp.
2.4.1.248 D662A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Paenibacillus sp.
2.4.1.248 E341A site-directed mutagenesis, the mutant is inactive Paenibacillus sp.
2.4.1.248 E341Q site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Paenibacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.248 Niallia circulans P94286 gene cit
-
2.4.1.248 Niallia circulans T-3040 P94286 gene cit
-
2.4.1.248 Paenibacillus sp. G9MBW2 gene cit
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.248 additional information isomaltotetraose is the smallest substrate for the CITase, and major product of recombinant CITase-598K is cycloisomaltoheptaose Paenibacillus sp. ?
-
 ?
2.4.1.248 additional information isomaltotetraose is the smallest substrate for the CITase, and major product of recombinant CITase-T3040 is cycloisomaltooctaose Niallia circulans ?
-
 ?
2.4.1.248 additional information isomaltotetraose is the smallest substrate for the CITase, and major product of recombinant CITase-T3040 is cycloisomaltooctaose Niallia circulans T-3040 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.248 CITase
-
 Niallia circulans
2.4.1.248 CITase
-
 Paenibacillus sp.
2.4.1.248 CITase-598K
-
 Paenibacillus sp.
2.4.1.248 CITase-T3040
-
 Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.248 45 50 assay at Paenibacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.248 40
-
 recombinant enzyme, stable up to Niallia circulans
2.4.1.248 50
-
 recombinant enzyme, stable up to Paenibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.248 5.5 8
-
 Niallia circulans
2.4.1.248 5.5 8
-
 Paenibacillus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.248 5 9 stable at Niallia circulans
2.4.1.248 5 9 stable at Paenibacillus sp.

General Information

EC Number General Information Comment Organism
2.4.1.248 evolution CITase is a member of the glycoside hydrolase family 66, GH66 Niallia circulans
2.4.1.248 evolution CITase is a member of the glycoside hydrolase family 66, GH66 Paenibacillus sp.
2.4.1.248 additional information catalytically important residues of CITase-598K are Asp144, Asp269, and Glu341 Paenibacillus sp.