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Literature summary extracted from

  • Wang, Y.; Niemi, J.; Airas, K.; Ylihonko, K.; Hakala, J.; Mäntsälä, P.
    Modifications of aclacinomycin T by aclacinomycin methyl esterase (RdmC) and aclacinomycin-10-hydroxylase (RdmB) from Streptomyces purpurascens (2000), Biochim. Biophys. Acta, 1480, 191-200.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.95 expressed in Streptomyces lividans TK24 Streptomyces purpurascens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.95 0.0155
-
 aclacinomycin T pH 7.5, 37°C Streptomyces purpurascens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.95 30000
-
 1 * 30000, SDS-PAGE Streptomyces purpurascens
3.1.1.95 31729
-
 1 * 31729, calculated from sequence Streptomyces purpurascens
3.1.1.95 33000
-
 gel filtration Streptomyces purpurascens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.95 aclacinomycin T + H2O Streptomyces purpurascens
-
 15-demethylaclacinomycin T + methanol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.95 Streptomyces purpurascens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.95
-
 Streptomyces purpurascens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.95 mycelium
-
 Streptomyces purpurascens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.95 169.4
-
 pH 7.5, 37°C Streptomyces purpurascens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.95 aclacinomycin T + H2O
-
 Streptomyces purpurascens 15-demethylaclacinomycin T + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.95 monomer 1 * 30000, SDS-PAGE Streptomyces purpurascens
3.1.1.95 monomer 1 * 31729, calculated from sequence Streptomyces purpurascens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.95 RdmC
-
 Streptomyces purpurascens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.95 37
-
-
 Streptomyces purpurascens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.95 30 45 30°C: 58% of maximal activity, 45°C: 87% of maximal activity Streptomyces purpurascens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.95 7.5
-
-
 Streptomyces purpurascens