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Literature summary extracted from

  • Tavares, C.D.; OBrien, J.P.; Abramczyk, O.; Devkota, A.K.; Shores, K.S.; Ferguson, S.B.; Kaoud, T.S.; Warthaka, M.; Marshall, K.D.; Keller, K.M.; Zhang, Y.; Brodbelt, J.S.; Ozpolat, B.; Dalby, K.N.
    Calcium/calmodulin stimulates the autophosphorylation of elongation factor 2 kinase on Thr-348 and Ser-500 to regulate its activity and calcium dependence (2012), Biochemistry, 51, 2232-2245.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.20 Calmodulin Ca2+/calmodulin stimulates the autophosphorylation of elongation factor 2 kinase on Thr348 and Ser500 to regulate its activity and calcium dependence. At a calmodulin concentration of 0.002 mM, the enzyme exhibits significant activity Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.20 S445A no difference in activity compared to wild-type enzyme with respect to the ability to phosphorylate a peptide substrate Homo sapiens
2.7.11.20 S445D no difference in activity compared to wild-type enzyme with respect to the ability to phosphorylate a peptide substrate Homo sapiens
2.7.11.20 S474A no difference in activity compared to wild-type enzyme with respect to the ability to phosphorylate a peptide substrate Homo sapiens
2.7.11.20 S474D no difference in activity compared to wild-type enzyme with respect to the ability to phosphorylate a peptide substrate Homo sapiens
2.7.11.20 S500A no difference in activity compared to wild-type enzyme with respect to the ability to phosphorylate a peptide substrate Homo sapiens
2.7.11.20 S500D no difference in activity compared to wild-type enzyme with respect to the ability to phosphorylate a peptide substrate. The mutation renders the enzyme Ca2+-independent Homo sapiens
2.7.11.20 T348A about 95% loss of the kinase activity Homo sapiens
2.7.11.20 T348D about 93% loss of the kinase activity Homo sapiens
2.7.11.20 T353A no difference in activity compared to wild-type enzyme with respect to the ability to phosphorylate a peptide substrate Homo sapiens
2.7.11.20 T353D no difference in activity compared to wild-type enzyme with respect to the ability to phosphorylate a peptide substrate Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.20 Ca2+ Ca2+/calmodulin stimulates the autophosphorylation of elongation factor 2 kinase on Thr348 and Ser500 to regulate its activity and calcium dependence. Half-maximal activity at 140 nM Ca2+. Maximal activity 0.003 mM Ca2+ Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.20 Homo sapiens O00418
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.20 phosphoprotein upon incubation with Ca2+ and calmodulin, the enzyme undergoes rapid autophosphorylation. Identification of five major autophosphorylation sites: Thr348, Thr353, Ser445, Ser474, and Ser500. Phosphorylation of Thr348 is required for substrate phosphorylation, but not selfphosphorylation Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.20
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.20 ATP + acetyl-RKKYKFNEDTERRRFL-amide
-
 Homo sapiens ADP + acetyl-RKKYKFNED(phospho)TERRRFL-amide
-
 ?
2.7.11.20 ATP + [elongation factor 2]
-
 Homo sapiens ADP + [elongation factor 2] phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.20 EEF-2K
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.20 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.20 7.5
-
 assay at Homo sapiens